fastq-multx support of custom dual-barcodes with variable length
61 views
Skip to first unread message
Eckart Stolle
unread,
Feb 12, 2015, 9:22:20 AM2/12/15
Reply to author
Sign in to reply to author
Forward
Sign in to forward
Delete
You do not have permission to delete messages in this group
Copy link
Report message
Show original message
Either email addresses are anonymous for this group or you need the view member email addresses permission to view the original message
to ea-u...@googlegroups.com
Hi
I was wondering of multx also works with customs barcodes (dual barcoding) whioch have variable length (5-10 nt)? They are introduced into amplicons and thus will be at the beginning of the sequence read (ie there will be no index reads). [Illumina MiSeq PE)
Thanks in advance Eckart
Erik Aronesty
unread,
Feb 24, 2015, 8:58:23 AM2/24/15
Reply to author
Sign in to reply to author
Forward
Sign in to forward
Delete
You do not have permission to delete messages in this group
Copy link
Report message
Show original message
Either email addresses are anonymous for this group or you need the view member email addresses permission to view the original message
to ea-u...@googlegroups.com
Should work fine. I have learned that mcf will not trim the second barcode off of the sequence read, however, and this needs to be fixed. IT should be a trivial source-code patch.