fastq-multx support of custom dual-barcodes with variable length

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Eckart Stolle

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Feb 12, 2015, 9:22:20 AM2/12/15
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Hi

I was wondering of multx also works with customs barcodes (dual barcoding) whioch have variable length (5-10 nt)? They are introduced into amplicons and thus will be at the beginning of the sequence read (ie there will be no index reads). [Illumina MiSeq PE)

Thanks in advance
Eckart

Erik Aronesty

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Feb 24, 2015, 8:58:23 AM2/24/15
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Should work fine.   I have learned that mcf will not trim the second barcode off of the sequence read, however, and this needs to be fixed.  IT should be a trivial source-code patch.
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