Mariu,
First question: In over 20 years of using E-Prime I have never heard of anyone using an animated .gif, and I think that you are the first to ask here. If I had an animated .gif file handy I would try it and see what happens. You could also ask PST Support directly (and then write back here to let us know their answer!).
Second question: Yes, E-Prime will happily use a mix of image file formats within a Procedure, or during different iterations of the same Procedure. You will of course need to supply the full file name, including extension (e.g., .jpg, .gif), each time.
-- David McFarlane