> 1/How do I recognize " b0 image acquired at the opposite phase encoding"? If I am correct, the bo image would be T2 with few images. I have such sequences but I do not find the phase encoding direction? If DSI Studio do not produce " xxxxx_dMRI_dir258_PA_SBRef.nii.gz" file means that I do not have the bo image?
It is likely that your sequence was not configured to acquire it.
To confirm, you may need to talk to the MR physicists who acquired the
data for you.
>
> 2/I want to only use DSI_studio and, if I am not mistaken, the use of FSL Topup is interesting but not a mandatory preprocessing tool. I do not have any bad slice according to DSI-Studio. How can I interpret the quality assessment made by DSI Studio (Fiber index)?
>
This video may help.
https://www.youtube.com/watch?v=stL4GMeTC1I&t=8s
You don't have reversed phase encoding b0, and thus FSL TOPUP cannot help here.
> 3/Nodular heterotopia can be quite small and difficult to see on raw DWI data. However, one patient suffers from shizencephaly and heterotopia: in this particular patient, it was easy to detect a flip along x-axis (even if it tooks me a while to understand how to correct it...). How can I be sure that all my patients do not have such a flip?
It is rare to have images flipped in x, and it is likely that you
don't have to do it.
Make sure you did not confuse neurology left-right convention to
radiology's convention.
>
> 4/It is unclear to me if I have to choose DTI or GQI: using GQI and automatic fiber tracking, a lot of tracks have not been reconstructed in my first round of analysis whereas with DTI all tracks have been reconstructed. More broadly, if I obtain correct tractography with DTI and GQI, which one should I prefer in case of a patient with a brain lesion? I think GQI is more appropriate since it is a model free method?
Both will generate very similar results in your case because you only
have 30 direction acquire in one b-value.
The differences will become more obvious only if you acquired 23+
b-values and hundred's of direction, just as recommended as here:
http://dsi-studio.labsolver.org/Manual/b-table-for-qbi-dsi-and-gqi-scans
>
> 5/It becomes clear to me that connectivity have to be perform with a very large amount of seeds. What is the correct way to determine the threshold? If I let it to a small value, I have a lot of noise in my matrix but I don't want to loose to much information.
>
I usually don't use connectivity matrix in my study, and I am sorry I
don't have a good insight.
My two cents is to check out a good study and see how they did it.
> 6/Some teams have performed CSD-based tractography for patients suffering from heterotopia. It is possible to perform CSD based tractography with DSI Studio?
>
Sure. Here's the instructions:
http://dsi-studio.labsolver.org/Manual/data-exchange-between-dsi-studio-and-mrtrix#TOC-Using-MRtrix-FOD-in-DSI-Studio
Best regards,
Frank
> Thanks a lot for all your help,
> Sincerely yours
> M.ZANELLO
>
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