Hi Anna,
For the cross-sectional analysis, the best approach is to create a single database that includes all subjects. You can then use the cohort selection feature to include or exclude specific groups as needed for your comparisons.
Regarding the pre- and post-intervention comparisons, I recommend first computing the paired differences using the [Step C2] interface. This will allow you to generate the necessary difference maps before proceeding with further statistical analysis.
I hope this helps clarify the setup. Please let me know if you have any other questions.
Best regards,
Frank