Dear Dr. Yeh,
I am working with diffusion MRI data from infants aged approximately 1–18 months, I am using DSI Studio to analyze the uncinate fasciculus (UF).
I generated UF tracts in two ways:
Using the DSI Studio neonate group template.
Using my own neonatal data reconstructed with QSDR into a human neonatal template.
My acquisition is single‑shell DTI with 15 directions at b=800 s/mm². Even when I apply AutoTrack with the UF atlas in template space, the UF rendered on my data looks noticeably different from the one on the DSI neonate template (more fragmented and with a different overall shape).
Could you please advise on the main reasons this discrepancy might occur (e.g., limitations of 15‑direction b=800 data, QSDR registration issues, or tracking thresholds tuned for the group template) and suggest parameter adjustments or best practices to obtain a more reliable UF reconstruction in this acquisition setting?
Best regards,
Shashank Bansal