Hi, Dr. Yeh! Thanks for looking into this. I neglected to mention I am on the latest GPU version of DSI Studio (installed 1/27/26). Here are the exact steps I took:
1) Go to R1L Linear Registration within the Tools tab of the GUI
2) Select ROI_MNI_V4.nii as the Subject Image
3) Select dsi_studio_win/atlas/human/human.QA.nii as the Template image
4) Click Refine in the Registration GUI
5) Click Run registration in the Registration GUI
6) Go to Files -> Save Transformed Image and save as ROI_MNI_V4.nii.gz
7) Add ROI_MNI_V4.nii.gz and ROI_MNI_V4.txt into dsi_studio_win/atlas/human/
8) Exit DSI Studio and restart DSI Studio
9) Enter Step T3: Fiber Tracking
10) Select the ROI_MNI_V4 atlas from the Atlas tab in step T3a: Assign Regions
At this point a progress bar will appear for a few seconds but remain at 0/100% and then the GUI will crash.