DSI Studio crash after adding ROIs from imported atlas

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Michael Houston

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Jan 30, 2026, 4:09:58 PM (5 days ago) Jan 30
to DSI Studio
Hello! I was following the steps in this tutorial (https://dsi-studio.labsolver.org/doc/gui_t3_roi_tracking.html) to add in the AAL atlas as a new atlas for region-to-region structural connectivity analysis. I successfully transformed the .nii file to the human.QA file and generated the accompanying .txt file with ROI names and added both the .txt and .nii.gz files into the ...\atlas\human path. However, when I try to add this atlas into step T3, DSI Studio attempts to read the files for a few seconds and crashes shortly after. I would greatly appreciate any advice on how to solve this :)

Frank Yeh

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Jan 30, 2026, 4:12:07 PM (5 days ago) Jan 30
to m.jamesh...@gmail.com, DSI Studio
Sorry about the bug.

Could you send me the files that can reproduce the crash?

(and also the exact steps)

I will look into it and fix.

Michael Houston <m.jamesh...@gmail.com> 於 2026年1月30日 週五 16:09 寫道:
Hello! I was following the steps in this tutorial (https://dsi-studio.labsolver.org/doc/gui_t3_roi_tracking.html) to add in the AAL atlas as a new atlas for region-to-region structural connectivity analysis. I successfully transformed the .nii file to the human.QA file and generated the accompanying .txt file with ROI names and added both the .txt and .nii.gz files into the ...\atlas\human path. However, when I try to add this atlas into step T3, DSI Studio attempts to read the files for a few seconds and crashes shortly after. I would greatly appreciate any advice on how to solve this :)

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Michael Houston

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Jan 30, 2026, 4:38:02 PM (5 days ago) Jan 30
to Frank Yeh, DSI Studio
Hi, Dr. Yeh! Thanks for looking into this. I neglected to mention I am on the latest GPU version of DSI Studio (installed 1/27/26). Here are the exact steps I took:

1) Go to R1L Linear Registration within the Tools tab of the GUI
2) Select ROI_MNI_V4.nii as the Subject Image
3) Select dsi_studio_win/atlas/human/human.QA.nii as the Template image
4) Click Refine in the Registration GUI
5) Click Run registration in the Registration GUI
6) Go to Files -> Save Transformed Image and save as ROI_MNI_V4.nii.gz
7) Add ROI_MNI_V4.nii.gz and ROI_MNI_V4.txt into dsi_studio_win/atlas/human/
8) Exit DSI Studio and restart DSI Studio
9) Enter Step T3: Fiber Tracking 
10) Select the ROI_MNI_V4 atlas from the Atlas tab in step T3a: Assign Regions
At this point a progress bar will appear for a few seconds but remain at 0/100% and then the GUI will crash.

Thanks,
Michael
--
PhD Student, Biomedical Engineering Dept.
University of Miami
MSBE '20, Biomedical Engineering Dept.
University of Houston

ROI_MNI_V4.txt
ROI_MNI_V4.nii

Frank Yeh

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Jan 30, 2026, 4:43:21 PM (5 days ago) Jan 30
to Michael Houston, DSI Studio
Thank you very much.
I will fix the bug and get back to you.
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