Plant Tissue Culture Research Papers Pdf

[Idara Viengxay]

PlantCell, Tissue and Organ Culture (PCTOC) highlights breakthrough technologies and discoveries in plant biology and biotechnology, spanning the field from new insights into the process of in vitro propagation to the conservation of plant biodiversity.Has been examining progress in plant biotechnology for over 40 years.Led by a recognized editorial team known for outstanding performance.Actively promotes published articles through various social media activities.Publishes timely Topical Collections on all aspects of plant cell culture and biotechnology.Accepts original articles, reviews, and perspective papers covering novel discoveries.

It is with great sadness that we learnt a few weeks ago that Prof. Dr. Geert-Jan de Klerk, who had also been an Editor-in-Chief of PCTOC, passed away on 8 February 2023.

Sergio Ochatt has written an Obituary, which you can freely access here

As a content and community manager, I leverage my expertise in plant biotechnology, passion for tissue culture, and writing skills to create compelling articles, simplifying intricate scientific concepts, and address your inquiries. As a dedicated science communicator, I strive to spark curiosity and foster a love for science in my audience.

Plant tissue culture utilizes small plant fragments, such as buds, leaves, or even single cells, to regenerate entire plants under controlled conditions. These specialized environments, often contained within glass vessels with a nutrient-rich medium, provide the ideal setting for growth and development. Essentially, it's like having a mini-greenhouse where specific conditions can be meticulously controlled to optimize plant growth.

Studying historical success stories in tissue culture can provide valuable insights into effective strategies and approaches. Conversely, analyzing past failures can help you avoid repeating mistakes and develop more robust protocols.

The paper offers valuable insights for beginner tissue culturists interested in the broader applications and potential of tissue culture technology. It focuses on plant cell culture technology and its potential to address global challenges like sustainability and limited resources. It specifically explores the use of plant cell culture to produce plant-derived substances for food and cosmetic products.

This chapter focuses on the specific design requirements for plant tissue culture laboratories, highlighting their unique needs compared to other types of labs. It offers guidelines for designing plant tissue culture labs, presenting various options to fulfill various needs.

So, if you want to build a tissue culture-based lab and want to learn about the structures and utilities, the layout of labs, different areas in the labs, equipment and materials required, etc, this article is for you!

Well, this isn't a research article, but you'll find a library of articles on this website on the subject of tissue culture. Whether you're interested in learning about the basics, its applications, or its uses, we have everything you need to get started. Additionally, we offer numerous articles covering the materials and equipment you need, building a lab under budget, and even occasional expert interviews! All the articles are meticulously researched, so you can be sure you'll find the answers to any tissue culture-related question you might have. Give them a try!

Embarking on a journey into plant tissue culture has never been more accessible and convenient. The promising growth prospects in the plant tissue culture market highlight the increasing relevance of this field. Whether you're a seasoned researcher or a budding enthusiast, the key to successful tissue culture lies in having the right equipment, media, and chemicals at your disposal.

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The aim of this review is to critically assess the benefits and limitations associated with the use of in vitro plant cell and organ cultures as research tools in phytoremediation studies. Plant tissue cultures such as callus, cell suspensions, and hairy roots are applied frequently in phytoremediation research as model plant systems. In vitro cultures offer a range of experimental advantages in studies aimed at examining the intrinsic metabolic capabilities of plant cells and their capacity for toxicity tolerance. The ability to identify the contributions of plant cells to pollutant uptake and detoxification without interference from microorganisms is of particular significance in the search for fundamental knowledge about plants. However, if the ultimate goal of plant tissue culture experiments is the development of practical phytoremediation technology, the limitations inherent in the use of in vitro cultures as a representative of whole plants in the field must be recognized. The bioavailability of contaminants and the processes of pollutant uptake and metabolite distribution are likely to be substantially different in the two systems; this can lead to qualitative as well as quantitative differences in metabolic profiles and tolerance characteristics. Yet, many studies have demonstrated that plant tissue cultures are an extremely valuable tool in phytoremediation research. The results derived from tissue cultures can be used to predict the responses of plants to environmental contaminants, and to improve the design and thus reduce the cost of subsequent conventional whole plant experiments.

Growth and morphogenesis of in vitro cultures of plant cells, tissues, and organs are greatly influenced by the composition of the culture medium. Mineral nutrients are necessary for the growth and development of plants. Several morpho-physiological disorders such as hooked leaves, hyperhydricity, fasciation, and shoot tip necrosis are often associated with the concentration of inorganic nutrient in the tissue culture medium. Silicon (Si) is the most abundant mineral element in the soil. The application of Si has been demonstrated to be beneficial for growth, development and yield of various plants and to alleviate various stresses including nutrient imbalance. Addition of Si to the tissue culture medium improves organogenesis, embryogenesis, growth traits, morphological, anatomical, and physiological characteristics of leaves, enhances tolerance to low temperature and salinity, protects cells and against metal toxicity, prevents oxidative phenolic browning and reduces the incidence of hyperhydricity in various plants. Therefore, Si possesses considerable potential for application in a wide range of plant tissue culture studies such as cryopreservation, organogenesis, micropropagation, somatic embryogenesis and secondary metabolites production.

Copyright 2014 Sivanesan and Park. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro culture is a method applied for the growth and development of plant cells, tissues, and organs that uses a nutritive culture medium under controlled sterilized conditions. This method is considered one of the most promising and environmentally friendly biotechnological practices for the sustainable supply of biofuels11. There are three main in vitro culture systems including organogenesis (e.g., embryogenesis, direct and indirect shoot regeneration), rhizogenesis, and callogenesis, as shown in Fig. 1. Among these methods, callogenesis can be considered a robust method for biofuel production. The callus is generally defined as an irregular bulk of parenchymatous tissue with meristematic cells that are broadly used for production of different bioactive plant molecules11. The main advantages of callus culture are:

Somaclonal variations, which are usually seen in callus culture, can result in changes to metabolic pathways and even allows the production of new metabolites. Any phenotypic variation during callus culture is referred to as somaclonal variation that can be a result of RNA interference, histone modification, chromatin remodeling, DNA methylation, and spontaneous mutation13.

Based on the above-aforementioned advantages, it can be concluded that callus culture has the potential to meet the exponentially growing demand for biofuel production in the near future. In this study, different parts of in vitro -grown industrial hemp seedlings were employed for callus production, and further used as a new generation of energy crop in the HTL process.

The results presented in Table 1 raise an interesting question regarding the changes in the chemical and elemental composition of samples. These changes can be related to somaclonal variation occurring during callus culture. This phenomenon can lead to changes in metabolic pathways due to RNA interference, histone modification, chromatin remodeling, DNA methylation, and spontaneous mutation13. Recently, Adamek et al. (2021), through whole-genome sequencing, showed that somatic mutation in cannabis can change the metabolic pathway of cannabinoid biosynthesis28. We believe that 5th generation energy crops could be even more improved through somaclonal variation and/or genetic manipulation to obtain a sample with the lowest amount of lignin. Since clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome editing and Agrobacterium-mediated gene transformation have been developed in cannabis, genetic manipulation of callus can be considered a promising tool to change the lignin pathways in order to produce more bioenergy29,30,31. This study suggests a new approach for future research projects that utilize genetically modified crops for biofuel production.

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