Re: [DIYbio] Purchasing DNA synthesizer - Question about BP build out

[Dan Kolis]

Hello 'k-101',

Independent of the hardware, how do you propose to append promoters, markers, tails, etc into the gene(s) desired ? Getting them expressed may be non-trivial...

Is there a Q.C. step that's understandable for intermediate results ?

Being specific to answer this may benefit you... Answering a question to others often clarifies it for yourself too.

ex: a Fat jupyter notebook full of files and notes, or what ?

Big projects are fun projects, you named yourself one.

Good luck !

my ref: 19 Aug 2022, DIYbio, NAFL, koeng101, Question about BP build out

 

[Koeng]

> Independent of the hardware, how do you propose to append promoters, markers, tails, etc into the gene(s) desired ? Getting them expressed may be non-trivial...

GoldenGate. Expression is outside of outside of scope, though it's kinda easy in my experience.

> Is there a Q.C. step that's understandable for intermediate results ?

Sequencing.

> Being specific to answer this may benefit you... Answering a question to others often clarifies it for yourself too.

I've cloned thousands of genes myself and was part of designing of many genetic toolkits (led https://stanford.freegenes.org/ for 3 years). Also a main contributor of Poly (https://github.com/timothystiles/poly), and founded a company doing full automation for synbio (in particular, the DNA assembly space). I've built functioning systems for this task before, which is honestly quite a bit more illuminating than talking about building them.

[karim bernardet]

Hi

With a good practice of Arduino/Raspberry pi, with a 3d printer and some knowledge of synthetic biology (and a huge motivation of course) would it be possible, for educational purposes, to build a machine that would synthesize a very small molecule of DNA ?

I had seen some prototypes on the IGEM site :
https://2015.igem.org/Team:Cooper_Union/Loomino_Description
https://2021.igem.org/Team:Aachen/Description
(they use enzymatic method)

DNA electrowetter below
https://forum.arduino.cc/t/dna-electrowetter-device/956082

(and assuming the reagents would be accessible...)

Cheers

Karim

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