Artificial Jellyfish

[Jeremy[biohak]]

Here's here's an article I posted up on my blog. Apparently researchers were able to produce this jellyfish by grown muscle cells along the outer surface of a polymer mold.

[Petfixer71]

Do you have Any experience with cell culture on extracellular matrix??

[Jeremy[biohak.net]]

No I do not. I wish I did. I don't really have access to the type of equipment necessary to accomplish that. I am interested though in maybe converting a rep rap to do sugar extrusions. Are the home cnc techniques that could work for something like this?

[Jordan Miller]

here's our sugar extruder:

http://blog.reprap.org/2012/07/on-challenge-of-3d-printing-sugar-for.html

cheers,

jordan

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[Cathal Garvey]

IMHO, Sugar would make a poor substrate for cell culture. The osmotic
pressure of growing on sugar would be huge; that's one reason why it's
traditional to add so much sugar to preserves like jam.

On 30/07/12 14:07, Jordan Miller wrote:
> here's our sugar extruder:
> http://blog.reprap.org/2012/07/on-challenge-of-3d-printing-sugar-for.html
>
> cheers,
> jordan
>
>
>
> On Jul 30, 2012, at 1:08 AM, "Jeremy[biohak.net]" <jerem...@gmail.com>
> wrote:
>
> No I do not. I wish I did. I don't really have access to the type of
> equipment necessary to accomplish that. I am interested though in maybe
> converting a rep rap to do sugar extrusions. Are the home cnc techniques
> that could work for something like this?
>
> On Thursday, July 26, 2012 8:50:59 AM UTC-5, Petfixer71 wrote:
>>
>> Do you have Any experience with cell culture on extracellular matrix??
>>
>>
>>
>> On Monday, July 23, 2012 3:07:40 AM UTC-4, Jeremy[biohak.net] wrote:
>>>

>>> Here's <http://www.biohak.net/node/12>here's an article I posted up on

>>> my blog. Apparently researchers were able to produce this jellyfish by
>>> grown muscle cells along the outer surface of a polymer mold.
>>>
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[Nathan McCorkle]

On Tue, Jul 31, 2012 at 4:59 PM, Cathal Garvey <cathal...@gmail.com> wrote:
> IMHO, Sugar would make a poor substrate for cell culture. The osmotic
> pressure of growing on sugar would be huge; that's one reason why it's
> traditional to add so much sugar to preserves like jam.
>

Cathal, they don't use the sugar to grow on, they print a mold with
the sugar, then add gelatin + cells and pour it over the mold. Once
the gelatin hardens, the mass is rinsed in an orbital shaker with
media or buffer (not sure which) to dissolve the sugar, then that
liquid is replaced with media. In Jordan's case, the mold looks like
vasculature to aid perfusion later (he perfuses with orbital shaking,
but I think he's also working on using a pump driven system).

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[Cathal Garvey]

Oh, that is *very* clever. My apologies, I was only running off what I
saw in the email thread! :)

On 31/07/12 22:04, Nathan McCorkle wrote:
> On Tue, Jul 31, 2012 at 4:59 PM, Cathal Garvey <cathal...@gmail.com> wrote:
>> IMHO, Sugar would make a poor substrate for cell culture. The osmotic
>> pressure of growing on sugar would be huge; that's one reason why it's
>> traditional to add so much sugar to preserves like jam.
>>
>
> Cathal, they don't use the sugar to grow on, they print a mold with
> the sugar, then add gelatin + cells and pour it over the mold. Once
> the gelatin hardens, the mass is rinsed in an orbital shaker with
> media or buffer (not sure which) to dissolve the sugar, then that
> liquid is replaced with media. In Jordan's case, the mold looks like
> vasculature to aid perfusion later (he perfuses with orbital shaking,
> but I think he's also working on using a pump driven system).
>

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