That Canadian company (or part of a uni) that was making a magnetic bead plasmid assembly kit

[Dakota Hamill]

Anyone remember their name?  I tried doing some googling and group searching but couldn't get anything back.

They were part of a university I believe, but did a small spinoff to sell assembly kits, sort of like bio brick assembly but I remember there being magnetic beads or resin beads of some type involved.

[Cory Tobin]

http://www.genomikon.ca/

[Dakota Hamill]

aww yisss  thanks

On Wed, Feb 19, 2014 at 3:41 PM, Cory Tobin <cory....@gmail.com> wrote:

http://www.genomikon.ca/

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[Cathal Garvey]

We had the kit in the Science Gallery's "Community Biolab" during "Grow
Your Own", I regret not having had time to try it out during my week
there. :)

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[Dan]

I used it at the FBI sponsored conference at BioCurious. It worked like a charm. LaBiohackers has taught two classes using the new kit. The only downside is you really are stuck with what is in the kit, which is good for a safety perspective, but you can't really make your own poly magnetic beads or easily order them cheaply.

> <0x988B9099.asc>

[Nathan McCorkle]

On Wed, Feb 19, 2014 at 1:05 PM, Dan <djwr...@gmail.com> wrote:
> I used it at the FBI sponsored conference at BioCurious. It worked like a charm. LaBiohackers has taught two classes using the new kit. The only downside is you really are stuck with what is in the kit, which is good for a safety perspective, but you can't really make your own poly magnetic beads or easily order them cheaply.

Dan, what do you mean by 'stuck with what is in the kit'?

Compared to the $400 genomikon kit, which I don't know how much
magnetic beads they include, these beads are less than that:
http://www.bioclone.us/DNA-RNA-conjugation-Immobilization-magnetic-beads-particle-resin-matrix.html
http://www.bangslabs.com/products/magnetic_microspheres
https://www.neb.com/products/s1419-oligo-dt25-magnetic-beads

You can get the oligo dT already attached, and require that your
starting biobrick or whatever the kids are calling DNA elements these
days has a matching stretch of As... or you can get the coupling kits
and attach your first strand directly to the bead:
http://www.idtdna.com/pages/docs/technical-reports/strategies-for-attaching-oligonucleotides-to-solid-supports.pdf?sfvrsn=4

[Cory Tobin]

> Dan, what do you mean by 'stuck with what is in the kit'?

The parts aren't easily manufactured. The overhangs are purified via
HPLC, so unless you have access to that you won't be able to make your
own parts. Doug (from Genomikon) is on this list I think, so maybe he
can provide insight about making Genomikon parts.

That being said, it's a great kit especially for educational purposes.
I've taught a couple classes using the kit and I think we've had 100%
success rate in terms of the students getting at least one colony
containing the correct plasmid. It's really foolproof and can be done
in a couple of hours.

I doubt it will be used in many research settings. If you're making
one-off DNA constructs you're better off using traditional cloning
methods. But I could see it being used in a situation where you have
a small number of parts that you recombine in lots of different
combinations, in which case it would save you a boat load of time.

-cory

[Nathan McCorkle]

Couldn't you just buy some primers of the same overhang and blunt ligate?

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[Kevin Chen]

Probably, although the efficiency would be lower because of self-ligation and then your parts that are attached to the beads could ligate to each other. Definitely a high risk of side products. Though it'd be nice if you could just take raw PCR product stick it in.

I can't remember what the concentration was for the DNA parts that they supply, maybe that's what gives such a high success rate? High purity from HPLC definitely helps, but I wonder if you could do it with just digested PCR product, maybe purified by a regular kit. That'd be important for using it in a research context. 

You could easily assemble expression vectors for your screening conditions when it comes to expressing or co-expressing recombinant protein. Right now, the vectors that my lab uses are completely static. It could have taken me a couple days to assemble all the plasmids that I've made so far vs. the weeks of work that I had put in.

Cheers,

Kevin