DIY restrictive enzymes

[Koeng]

Hello all! I was recently looking at restrictive enzymes and wanted to know if 1 there are any places that offer restrictive enzymes for residential addresses- and 2 if anyone knows a protocol for DIY restrictive enzyme purification. I know that edvotek has a kit, but does anyone have any experience with it? thanks in advance!

Koeng

[Nathan McCorkle]

what country are you in?

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-Nathan

[Tom Randall]

On Friday, February 22, 2013 12:19:30 AM UTC-5, Koeng wrote:

Hello all! I was recently looking at restrictive enzymes and wanted to know if 1 there are any places that offer restrictive enzymes for residential addresses- and 2 if anyone knows a protocol for DIY restrictive enzyme purification. I know that edvotek has a kit, but does anyone have any experience with it? thanks in advance!

Koeng

Promega (www.promega.com) has sent restriction enzymes to my residential address in the past, they are on par with NEB in terms of their selection and quality. Shipping is pretty high though.
Tom

[Koeng]

US. And I will check out promega. 

[Nathan McCorkle]

On Fri, Feb 22, 2013 at 7:49 AM, Koeng <koen...@gmail.com> wrote:
> US. And I will check out promega.

I won $500 in free stuff from promega just by letting them spam my
inbox with slightly interesting reagent ads for a year or two. Great
company! Their restriction enzyme list was pretty easy to scrape too
with python, so I could use it to optimize my cloning experiment for
price.

> https://groups.google.com/d/msg/diybio/-/QPTkRm4thF8J.

[Tom Randall]

On Friday, February 22, 2013 10:49:04 AM UTC-5, Koeng wrote:

US. And I will check out promega. 

I should have asked what country, forgot. I certainly dont know if this will work outside the US, and the shipping (on dry ice) might be prohibitive if they do. Am pretty sure they have non-US branches but would not know what their policies might be on shipping to residential addresses in the EU or elsewhere. Worth a try, I usually try every company I come across that has some interesting products just to test who is naughty (wont ship) and who is nice.