Which online store to procure yeast (for culturing) for small to medium quantities?

[AKS]

Hi DIY-Bioers

I wonder where, which online store to look for to procure yeast (for
culturing them)
in small to medium quantities? I can google but I would like to hear
your advice.
Thanks!

Sincerely

Aung

[Nathan McCorkle]

Which traits or strains do you desire? Most super-markets have yeast in packets.

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-Nathan

[Mega [Andreas Stuermer]]

If you don't want GFP yeast you can easily buy them in local stores :)

[Mike Horwath]

Homebrewing stores in the US, either online or brick, have a huge variety of yeast.  They will even have some non-saccharomyces species (mostly bretanomyces).

If you want published laboratory strains you probably have to go somewhere like ATCC.org, although they may not ship to a home address.

Mike

[Sebastian Cocioba]

Openbiotech.com sells yeast too. Sequenced strain.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

---

From: Mike Horwath
Sent: 2/10/2014 10:40 AM
To: diy...@googlegroups.com
Cc: aung...@gmail.com
Subject: [DIYbio] Re: Which online store to procure yeast (for culturing) for small to medium quantities?

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Learn more at www.diybio.org
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[Patrik D'haeseleer]

On Monday, February 10, 2014 7:45:59 AM UTC-8, Sebastian wrote:

Openbiotech.com sells yeast too. Sequenced strain.

Hm - don't see it on their website though.

Patrik

[Patrik D'haeseleer]

If you're looking for something a bit more specific than plain old baker's yeast, check out the page on educational culture suppliers I collected:

http://biocuriousmembers.pbworks.com/w/page/61714006/Culture%20collections

I was focusing primarily on bacterial species, but most of these have fungi and yeasts as well.

Patrik

[Cathal Garvey]

You rock, Patrik!

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[AKS]

Hi mates

Thanks for the answers and appreciated. Cheers!

Sincerely

Aung

[Hiro Protagonist]

Hi all,

I am interested in research with yeast, however it would be necessary to use a sequenced strain, which is available for private usage.

As it turned out one can actually get Saccharomyces cerevisiae stains (which would be my model organism of choice), however it appears that lab strains as S288c (which again would be the preferred strain) somehow seem to be classified as level 1 GMOs making them unavailable for non-institutational usage. 

Is there still any way to get access to these strains or does anyone know a fully sequenced strain of yeast with high homology to human proteins (especially mTOR), which doesn't require the customer to have access to level 1 biosafety labs?

Thanks in advance

[Scott]

The Odin has the W303 Saccharomyces cerevisiae strain which shares >85% of its genome with S288C:

http://www.the-odin.com/saccharomyces-cerevisiae-strain-w303/

No doubt as community biolabs mature they will have collected frozen stocks of a great many strains of BSL1 microorganisms. Of course, there is the overhead of maintaining and distribution but I think the community as a whole would benefit from getting access to these repositories as some nominal cost to make it worthwhile. Academic labs routinely share (officially and un-officially) lab strains and plasmids. I'd love to hear what the DIYbio community feels about this.

Cheers,

Scott

http://www.opensciencenet.org

[Josiah Zayner]

We also have BY4742(http://www.yeastgenome.org/strain/BY4742/overview) which is almost identical to S288C. Just email me if you are interested in obtaining it.

[Koeng]

S288c is a diploid, and the 2 haploids made when sporulating were BY4741 and BY4742. I have BY4741, a haploid of S288c. Email me privately if you need it. Josiah's BY4742 is probably fine for your application though. 

I'm currently working on a small DNA repository. It's taking a while because I'm optimizing all the protocols and plasmids to be extremely simple to use. I'm planning on packaging the DNA of the repository in phage, which costs nearly nothing to make and is stable for long periods at room temperature, which is nice for storage and large scale distribution. My current target organisms (ones which will have well characterized base vectors) are E coli, S cerevisiae, and A thaliana. It'll mainly be set up so people can contribute CDSs while I create/handle characterization and standardization of the basic plasmids. The idea is to distribute a 'base package' for each model organism with a sample of it. Each package includes all the basic vectors a person might need (characterized promoters, variety of markers). To put a gene in, you mix the CDS plasmid and a base package vector for an hour at 37c (with enzyme) and transform into a specific strain. That strain will be able to directly conjugate into whatever organism you are modifying.

I've finished the main base plasmid, just adding in the F1 origin for packaging this week, and hopefully be testing packaging by next week. It'll be a while until the full version is out since the A thaliana portion will take a while (characterizing method to directly conjugate with Agrobacterium and select on soil, avoiding Agrobacterium transformation and tissue culture) and I am also working out the exact efficiency details of using small synthetic DNA fragments in the goldengate reactions (to make the system a viable alternative to most other cloning methods)

-Koeng

[Patrik D'haeseleer]

Adding some info that I just received from Carolina.com on which exact yeast strains they sell:

We have 156250A Saccharomyces cerevisiae, an isolate from ATCC 9763. The ATCC site gives this information.

Saccharomyces cerevisiae Hansen
Classification	Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations	NRRL Y-567 [CBS 2978, CBS 5900, CCY 21-4-48, CCY 21-4-54, NCTC 10716, NCTC 7239, NCYC 87, Pattee 6, PCI M-50]

Additionally, we have 156251 Saccharomyces cerevisiae ellipsoidius, an isolate from ATCC 834. Apparently there is little information available on this variety.

[AKS]

Thanks and appreciated!

On 5/18/16, Patrik D'haeseleer <pat...@gmail.com> wrote:
> Adding some info that I just received from Carolina.com

> <http://www.carolina.com/browse/product-search-results?Ntt=cerevisiae> on

> which exact yeast strains they sell:
>
> We have 156250A Saccharomyces cerevisiae, an isolate from ATCC 9763. The
> ATCC site gives this information.
>

> *Saccharomyces cerevisiae* Hansen
>
> *Classification*

>
> Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae,
> Saccharomycetaceae, Saccharomyces, cerevisiae
>

> *Strain Designations*