RE: [DIYbio] Any "Genetic Engineering for Dummies"/ Getting

[Sebastian Cocioba]

Started tutorials out there?
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Cathal, you gave me some inspiration to really get this plant PEG
transformation thing down packed and turn it into the "my first GMO"
kit of sorts for plant biotech. Granted the regulations for eukaryotic
are much crazier and creating your own modified plant may cause some
ruckus if the species is weedy or invasive in any way. Even if there is
no realistic way of making it into a kit so beginners, or maybe the
intermediate amateur biologists can hack a plant. I see the main
limiting factor is sourcing agrobacterium. Hopefully it will get
deregulated soon but until then just crossing state lines with the
bugger is a felony. Until then I would rather just use PEG and develop
a near foolproof protocol anyone can use. Thanks for putting in all the
effort in developing your great looking kit. I really hope it reaches
goal soon. Im saving up to front some capital towards the campaign.
Best of luck to you and keep up the fantastic work! :)

PS my apologies for going off topic.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Cathal Garvey
Sent: =E2=80=8E1/=E2=80=8E28/=E2=80=8E2014 6:45 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] Any "Genetic Engineering for Dummies"/ Getting
Started tutorials out there?
I've seen this question so many times I've decided to answer it in the
most comprehensive way possible. See the crowdfunder in my signature. I
promise to make it as trivial as possible to make your first Genetically
Engineered Bacterium, and for it to be hackable so you can build
projects on top of it, too. :)

-Cathal

On 27/01/14 18:10, Joshua Hernandez wrote:
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> I've looked through a few of the existing "Getting Started" topics here,=
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> but they require a level of knowledge that is beyond me right now.
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> I was wondering if there are any tutorials, whether typed or in video, th=
at=20
> can teach genetic engineering to someone that know a little biology at ma=
x.
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> Like how Khan Academy can teach/explain to you clearly a topic that you=
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> know nothing about, is there a person/people who has/have does the same f=
or=20
> genetic engineering?
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> Videos or writings that you can follow along in little=20
> experiments/demonstrations that help new people learn and grasp this fiel=
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> without spending hundreds or thousands of dollars in tuition fees.
>=20

--=20
Please help support my crowdfunding campaign, IndieBB: Currently at
16.4% of funding goal, with 44 days left:
http://igg.me/at/yourfirstgmo/x/4252296
T: @onetruecathal, @IndieBBDNA
P: +3538763663185
W: http://indiebiotech.com

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[Cathal Garvey]

I hope it works out too, and sign me up for a plant kit ASAP! :)
Funny, the regulations in Europe for bacteria are the harshest, for
plants second, but oddly enough the GM regulations for animals are
minimal. You need to prove you can contain them, but there are no fees.
I think the reasoning is "if you can shoot it, you can contain an
outbreak". ;)

PEG transformation for plants sounds awesome; I'm always hoping to
migrate upwards to plants, but the requirement for a A) Gene gun or B)
Agrobacterium (class 2) nixes that idea.

That said, I've seen some photos of an *awesome* backyard plant
transformation lab using a DIY helium cannon for gene-gun style
biolistics, and apparently they're having some successes!

Still want to see how big a splash an "Open Source" Bt/RR crop variety
would make, particularly in defusing anti-GMO propaganda that's
predicated on the idea that only Monsanto can make GMOs, and that
farmers can never own the seed. :)

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