Refrigerated Centrifuge Alternatives

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Josh McGinnis

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May 19, 2021, 7:41:25 PMMay 19
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I'm in need of a refrigerated centrifuge for my agrobacterium electroporation experiments, but they're difficult and expensive to come by - even used on eBay. They're also super heavy and so shipping is also cost prohibitive. 

I'm considering buying a small mini-fridge and just running a small microcentrifuge inside of it after cooling it down overnight.

I don't think it'll be as efficient as most refrigerated centrifuges have coils tightly wrapped around the centrifuge chamber, but it'll def. be better than nothing.

Has anyone tried this or any other alternatives? 

Best,

Josh

Keith Warner

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May 19, 2021, 11:34:35 PMMay 19
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Do you have access to a coldroom?

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Srivatsa Aithal

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May 19, 2021, 11:34:44 PMMay 19
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Hey Josh,
   I do not know the volumes that would be used for centrifugation. 1.5 mL tubes could be centrifuged by placing them inside 50 ml tubes (bigger tubes) along with crushed ice as long as the duration is not very long. The 1.5Ml tubes could also be parafilm sealed.
  Not an ideal solution but could work.

Otherwise, I would buy a mini centrifuge and use it in a fridge as you described.

Thanks,
Sri

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Josh McGinnis

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May 20, 2021, 2:07:55 AMMay 20
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No, I work in my home lab.

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Josh McGinnis

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May 20, 2021, 2:08:03 AMMay 20
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Thanks Srivatsa - that's a great idea. 

ATG start

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May 21, 2021, 10:05:42 AMMay 21
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I'm interested by your solution, if you have have something working to avoid a refrigerated centrifuge, let us know ;) On my side, I would like to test just the tube on ice before centrifugation...

Koeng

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May 21, 2021, 11:58:51 AMMay 21
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https://www.nature.com/articles/srep24648

Not sure if this works with agro

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