Free fragment analysis/capillary eletrophoresis data analysis software

[Dorif]

Recently, I've released FragalyseQt v 0.4, codenamed "Jeffreys", now with support for fragment sizing! It is a free (free as in "freedom", not in "free beer" - Richard Matthew Stallman) software for fragment analysis/capillary electrophoresis data handling.

FragalyseQt supports sizing using either linear, cubic or 5th degree spline or LSQ of 2nd, 3rd or 5th order, can handle ABIF files from virtually every CE machine, even from ancient ABI 310 with ancient version of ABIF, not described in specifications published by ABI or RapidHIT ID, implementing only very limited subset of ABIF, or even HID files from ABI 3500 HID. It can export analysis results in CSV, or if internal analysis results are available (for files obtained using ABI 3500, SeqStudio, SeqStudio Flex) - it can export them too.

FragalyseQt is non-invasive analysis tool, leaving analysed files in exactly the same condition they were before analysis.

It is highly portable and proven to work at: Linux, macOS, Windows (Win7+), OpenBSD, FreeBSD, NetBSD and uses only 200 MB or RAM. It also supports Non-Latin run anmes and different interface languages as: English, Russian, French, Romanian, Bulgarian, Ukrainian. Language is set automatically using OS language settings.

Software is licensed under AGPL v3.

At the screenshot you may observe calculaed sizes of GlobalFiler allelic ladder peaks (in program window) and expected sizes with accepted deviations (in LibreOffice).

FragalyseQt source code is available at: https://github.com/Dorif/fragalyseqt

Release 0.4 is available at: https://github.com/Dorif/fragalyseqt/releases/tag/jeffreys_initial