Fwd: Protocols - reusing qiagen miniprep columns up to 10x

[Mackenzie Cowell]

Really cool.  Could be a $$$ saver.  Email me if you want a copy of the PDF.

Mac

---------- Forwarded message ----------
From: Kim de Mora <kim.d...@gmail.com>
Date: Thu, Nov 5, 2009 at 10:19 AM
Subject: Fwd: Protocols
To: Mackenzie Cowell <maco...@gmail.com>


Yo dude,

  Interesting stuff from Karmella including how to reuse qiagen colums.  Cheers!

Kim

---------- Forwarded message ----------
From: Haynes, Karmella A. <Karmell...@hms.harvard.edu>
Date: 2009/11/4
Subject: Re: Protocols
To: Kim de Mora <kim.d...@gmail.com>

Hey Kim,

1. Attached is the publication about regenerating Qiagen columns. Also, here is a link to the protocol I wrote for Davidson College:
http://www.bio.davidson.edu/courses/Molbio/Protocols/QIAQuick_recycle.html

2. Below is a reference for a paper about running gels at crazy-high voltages (I cant seem to access the paper online). It worked for me at Washington University, but not at Davidson. I think perhaps a circulating gel box is required (had one at WashU, not at Davidson)...

Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media.
Brody JR, Calhoun ES, Gallmeier E, Creavalle TD, Kern SE.
Biotechniques. 2004 Oct;37(4):598, 600, 602

3. Here’s a Davidson web page link to info about capillary gel electrophoresis: http://www.bio.davidson.edu/courses/genomics/method/Capillary.html

I hope this is helpful.

Best,
---Karmella

On 11/4/09 10:37 PM, "Kim de Mora" <kim.d...@gmail.com> wrote:

Karmella,

  This is just a quick follow up mail to ask about the protocols to use miniprep columns up to 10 times.  I was also wondering if you could send me a reference regarding the DNA capillary sequencing/PCR.  I'll put the idea to my mates and see what they can come up with.  Cheers!

Kim

--
Mac Cowell
DIYbio.org
p: 231.313.9062
w: has100ideas.com
tw: @100ideas

[Tito Jankowski]

On a related note, the Pearl Gel Box now includes a free sample of Faster Better Media buffer (made by the guys from the "Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media" publication) in a limited supply of orders. It runs much cooler at voltages that would normally melt a gel. It also cuts down on fogging of the lid.

Tito