How to get surface proteins from one bacteria to another? Can somebody help?

[Nils Reither]

Hi
I wonder if it is possible to get the genes from the O and H antigen proteins in Salmonalla bacteria transferred to more harmless bacteria such as Bacillus subtilus or Lactobacillus.
This way a risk free immunisation would be possible.

Cheers

Nils Reither, veterinarian

[Cathal Garvey]

While that woulda make an interesting project, I don't feel it's appropriate for 'DIYbio', as we generally avoid projects dealing with potential or known human pathogens. Not only is it illegal in most places to do this without a license, it's genuinely a bad idea to do it without professional training.

Not only that, it's be hard! You won't be able to get those genes synthesised because they're clearly derived from a pathogen. When the synthesis company run a BLAST on it and see 'salmonella', you're unlikely to convince them to make and ship it to you. That means you'd be stuck working with the pathogen itself, not a very friendly situation to be in.

I know someone on this list is looking at making vaccines to a common lizard-killing pathogen- fascinating project idea as no known cases of human infection have occurred. Provided one was careful, that would seem a good starting point for a more 'DIYbio' project.

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[Matthias Bock]

Any living organism injected, will cause
an immune response, which may go terribly wrong: e.g. sepsis
Therefore you would have to kill the bacteria anyways
before injection.

It would be more suitable to purify the H and O antigens
from a bacterial culture and inject them with some PAMP.
But I guess, these proteins have a high variation,
else the immune system wouldn't have a problem with them.

And:
You could accidently produce e.g. a pathogenic Bacillus
subtilis by these means.
Also you would probably need to handle a pathogenic Salmonella.
Therefore your undertaking would require an
official permission e.g. in Germany (GenTG ￜ8).

Cheers, Matthias

[Jeswin]

On Sun, May 27, 2012 at 11:57 AM, Matthias Bock <ma...@matthiasbock.net> wrote:
> And:
> You could accidently produce e.g. a pathogenic Bacillus
> subtilis by these means.
> Also you would probably need to handle a pathogenic Salmonella.
> Therefore your undertaking would require an

> official permission e.g. in Germany (GenTG §8).
>
I assume Dr Reither is working in a commercial lab and probably just
scouring for ideas in the area of animal vaccine. But for anyone else,
these disclaimers can't be ignored.

Anyway, I find it interesting that professional researchers are using
the DIYbio network. It shows that DIYbio is a haven for discussing
novel ideas.

[Mega]

It may also be possible to do it the other way round: put the chromosome of a harmless bacterium into such a cell. But wehen it multiplies, it will probably /surely develop it's natural lipid layer / membrane