Jason Bobe--
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This is *excellent* news, I'm delighted to see it!
Although I'm delighted at the web-interface (I don't feel the mailing list is the answer to everything), it would be nice to have the experts read even the digests of the mailing list from time to time, too. Their expert input would be very welcome on this list!
Any idea when we might see some of the first answers coming out? Or how long you expect the typical response time to be?
A huge THANK YOU for this service - this is an outstanding resource!
On Sat, Jan 19, 2013 at 6:44 PM, Jason Bobe wrote:
> Sort of like asking my doc to skim my email making to make sure I'm healthy
> :)
What's wrong with a doctor replying to his patient's emails, besides
liability/insurance? It might not be as absurd as you think.
On Jan 20, 2013 12:06 PM, "Jason Bobe" <jaso...@gmail.com> wrote:
>
> Yeah, my plan was to post the responses to the mailing list simultaneously with posting them in an archive at DIYbio.org. Maybe with a standard subject line prefix, like [DIYbio.org ABE] so they are easy to find.
>
Cool, sounds good!
Question:
We have a group at BioCurious that wants to print tissues using a bioprinter they have created - a machine that prints cells on a surface like an inkjet printer. They originally wanted to print mammalian tissue cells. We've decided not to allow this at BioC as we would want to treat such work as BSL2 and don't have those facilities (reason being there is no way to certify it is free of all human pathogens or that even if free the cell line would not become contaminated). I suggested to the group that they look at invertebrate cells, they said this was not suitable, but proposed using an amphibian or reptilian cell line. I can't think of why this wouldn't be safe, but as this is so far out of the work I have done I don't feel comfortable making that determination. Do you have any insight on if there are any safety considerations to working with amphibian or reptilian cells?
BioCurious is a community lab in the San Francisco bay area - you can find more about us at http://biocurious.org.
January 28, 2013
Response from Biosafety Advisory:
Work with amphibian or reptilian cells can be conducted safely at BSL-1. While these types of cells are less likely than mammalian tissue cells to carry human pathogens, if the cells will be isolated in the laboratory, as opposed to being purchased from a vendor, the group should be aware that fungal and bacterial skin infections could occur from contact with reptiles or amphibians (Lewis et al. Clinical Infectious Diseases 2003; 37: 390-397). Good work practices such as wearing gloves when working with the cells, especially if group members have broken skin, and washing hands after working with the materials should be followed.
The
group will want to devise a method to decontaminate their bioprinter after use
with the cell lines. An effective
disinfectant is a 1:10 dilution of Clorox bleach in water (1 part Clorox household
bleach – not scented or splashless versions – to 9 parts water). The surfaces/items to be decontaminated
should remain wet with the 1:10 dilution of bleach for at least 10 minutes to
make sure that they have been properly disinfected. Depending upon the materials used to build the bioprinter,
this bleach solution may cause corrosion of metal parts over time if the
equipment is not thoroughly rinsed with water.
Cool! First response is up, and it's about the idea of using amphibian or reptilian cell lines on the BioPrinter at BioCurious (for the record, we're not working with *any* animal cells right now):
GOOGLE TRANSLATION:
"Will you be able to ask questions in languages other than English?,
As the Spanish or French, the most popular at least"
Uhh yeah...I should have 30 points substracted from my IQ score I think. I remember looking at the page a long time ago when there were only a few answers, and checked back now to see the same page...and completely failed to nice the "see more" link. Gone too long without sleep, I think. (*Fake excuse*)
Apologies for polluting the interweb, everyone. And thanks to the panel for answerring my question!
Thanks, Jason!
I have to admit I didn't see the "More" button on the page for a long time either. You might want to rearrange things such that the most recent answer shows on top, rather than at the bottom.
Any more answers in the works? It's been a couple months since we saw the last one...
On Sun, Feb 9, 2014 at 12:41 AM, Patrik D'haeseleer <pat...@gmail.com> wrote:
Any more answers in the works? It's been a couple months since we saw the last one...
Indeed, more answers are in the works. Back in October, I gave a presentation at the ABSA national meeting and I'm happy to say that the biosafety advisory membership now includes 14 volunteers (up from 3 experts in the pilot).
February 18, 2014
March 8, 2014
March 10, 2014
Hi!
I was wondering. When I get my construct synthesized that makes bees resistant to viruses. Either I use crispr or siRNA - all the methods require a reverse complementary sequence to an essential viral protein.Now, gene synthesis orders are screened not to contain (human?) pathogens, and it is good that they do screen to prevent abuse.
However, I don't want to be put on a blacklist and maybe get troubles at the airport when travelling to the USA. I heard of a couple in the media who were not allowed to get onto their plane and travel to the USA. Noone refunded their flight tickets. They were just average guys, retired workers. Now immagine, you might get trouble when you're on a blacklist...
Do you know more about this?
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If you submit this directly to http://ask.diybio.org/ it'll go straight to our experts!
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