Hey guys!
I am currently working on a system in Bacillus and need a way to induce excision of some DNA... So I wanted to know if anyone has some DNA that I can PCR the Cre recombinase off of. I could do some plasmid trades, I have pGLO, pUC19, pMAL c5e, and OpenBrick Amp (a custom vector that is biobrick compatible and you can sequence it with M13 primers) I also have some Bacillus strains, 168, wild type (transformable), one with inducible competency, and one that glows green when sporing. If non of these you need, then I can also just pay you with, lets say, paypal.
Thanks!
-Koeng