Finally found a sample of Nostoc Commune!

[Mega]

http://en.wikipedia.org/wiki/Nostoc_commune

Hi,

I have been looking for this quite some time, and finally found it on a wet concrete wall - in my garden!!!


The wikipedia article says it can survive harsh conditions, repeated freeze-thaw cycles, has a pigment that protects it from UV radiation, it can desiccate (stop metabolism then)  and revive again under wet condition. It can also fix nitrogen from the air. Awesome! And - last but not least it's photosynthetically active.



Now that I have a sample, I could do some experimnts. Do nostoc (cyanobacteria) react to ampicilline?
Would a default transposon (which I may be getting) work with them?

Best,

[ruphos]

That stuff sounds pretty neat. Interested to hear what you find.

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[Ravasz]

Ampicillin works fine on spirulina, therefore I would assume it effects Nostoc too.

[Eugen Leitl]

On Sun, Apr 21, 2013 at 03:13:25AM -0700, Ravasz wrote:
> Ampicillin works fine on spirulina, therefore I would assume it effects
> Nostoc too.

Are there good transfection protocols for spirulina?

[Mega]

I found some transformation protocols. They range from just incubation with DNA to sonication and electroporation... 

However, I guess a pUC origin will not replicate in Cyanobacteria, right?? 

[jarlemag]

How did you identify it?

- J

[Mega]

Just looked at it closely. Will take it to university today, and have a close look with the microscope at it. 

However I'm positive about it... One sample dried out over the night (unintentionally), the next day I put water on it and it became green again. No plant will do that!

[Mega]

 

So, had a microscope view on it...

 

Pretty much looks like they were some kind of filamentous Cyanobacteria. Thus it is plausible that they are nostoc. And because of the shape, they *must* be N. Commune....

[Dakota Hamill]

Awesome pictures! Imagine things like that being the only things
floating around in the ocean billions of years ago. Cool stuff.

[Andreas Sturm]

>Imagine things like that being the only things
floating around in the ocean billions of years ago.

Yeah, awesome!! 


And as far as I rad, they would be perfect candidates to live on Mars... They can dry out and be revivied, survive UV-Radiation, do Photosynthesis, ...... Just, that they aren't glowing.... Yet.... 

On Tue, Apr 23, 2013 at 6:20 PM, Dakota Hamill <dko...@gmail.com> wrote:

Awesome pictures!  Imagine things like that being the only things
floating around in the ocean billions of years ago.  Cool stuff.

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