CRISPR in plants

[Tom De Medts]

Hi DIY-Bio DIYers,

I am considering delving into CRISPR for applications in plants.

For those who are experience in CRISPR work, here are my questions that I hope to get guidance for:

1. What is a good list of essential CRISPR resources I need to procure before actually designing experiments and conducting them? (For eg. vectors etc.)

2. Since I operate out of a garage lab in Tidewater area of Virginia, USA (the 757 MakerSpace does not appear to have such resources) from who do you think I can request / purchase items from your list (in answer 1)?

3. I have some limited bioinformatics experience. What are good online / offline resources to train myself in bioinformatic design of CRISPR probes?

4. A lot of the research papers on CRISPR arrays appear to be for mammalian species and related model species - not so much for plants at all. What are those reasons? Purely monetary, or any science-based limitations (like cell wall barrier to delivery, etc.)?

Thanks, in advance.

Best,

Tom

[Vanessa Lorenzo]

Hey Tom,

CRISPR for applications in plants, insteresting! I am in a similar path. Did you get any feedback?

Best

Vanessa