native colour / fluorscent pigments in E coli

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Mega [Andreas Stuermer]

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Nov 4, 2014, 5:08:58 PM11/4/14
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Hi everyone, I came across this... 

http://link.springer.com/article/10.1007%2FBF00446670#page-1

I was wondering, by just overexpressing tryphtophanase, can I turn E coli orange? 

As most of you probably heard of, genetic engineering here in Europe is heavily restricted. Using a native E coli gene does not fall under the GMO regulation (this exemption is called "self-cloning"). So everyone could legally engineer E coli in his basment here in Europe (though only add the orange pigment). Plus the pUC vectors are also exempted. 

What do you think of it? Worth trying? Or do you know other native E coli genes for colour? 


Mega [Andreas Stuermer]

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Nov 4, 2014, 5:28:27 PM11/4/14
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Ah, why so complicated? 

Riboflavin is bright yellow! If I find a bottleneck... yellow colonies?  

Mega [Andreas Stuermer]

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Nov 4, 2014, 5:31:16 PM11/4/14
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Darn. Yellow pigment on yellow agar. 

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