Re: [DIYbio] Digest for diybio@googlegroups.com - 4 updates in 1 topic

[Michael Crone]

On your original question. It is no longer maintained so you’ll have to reach out to the network to get any genes that you might want. (Under the listing of each page it shows you who already has the gene).

Best wishes

Michael

On Wed, 28 Dec 2022 at 05:17, <diy...@googlegroups.com> wrote:

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• Is BioBricks Free Genes Project still maintained and alive? - 4 Updates

Is BioBricks Free Genes Project still maintained and alive?

Dan Kolis <dank...@gmail.com>: Dec 27 09:13AM -0800 *Is this summary correct ?*   " The aspiration of many people in bio sciences that electronics provides macro concepts useful for application of nucleotide sequences is essentially incorrect. "   " These notions, ( that simplify life processes ), cannot be excised from viable life systems, including chaperones, post translation mRNA edits, long standing methylization attachments, and literally *hundreds of biological effects* implemented in life as built by Mother nature, in everything alive. "   Finally: "The reused terminology and desire to make VLSI like IDE's, ---may be useful in development of synthetic biology, but it appears this is almost simply a dead end. "   Is this possibly correct ?   Regards, Daniel B. Kolis   PS I saw paper saying the actual wet-lab attempts with BioBricks DB plopped into glassware had a success rate by the applicable goals of each project of around 2%. My belief system is that is not zero... But the notion itself needs a full scale downgrade from first principles. What replaces it is: *MORE USUAL HARD WORK AND NEW IDEAS ENTIRELY*.   what do you think ?   Daniel B. Kolis

Eric <zhigua...@gmail.com>: Dec 27 02:58PM -0500 molecular cloning in itself as a molecular biology technique is automatically regarded to some/most as 'biobricks' activity but its use far predates the synthetic biology movement

Dan Kolis <dank...@gmail.com>: Dec 27 12:59PM -0800 > I thought the biobricks 'story' is a uniform 'design' of promoters and so >> on, so gene expression of novel organisms includes adding protein >> expression with a cookie cutter uniform approach.   I'd guess this involves protocol so the urge to fulfilment is with a predefined approach; ex cut with a certain R.I. add this or that to detect which cells 'took', etc.   Not the major contribution of them, then ?   Isn't something akin to mail order mitosis, in in itself, barely worth a yawn ?   Daniel B. Kolis

Abizar Lakdawalla <abi...@gmail.com>: Dec 27 05:51PM -0800 are Dan Kolis answers from a random sentence generator?

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