Light Bulb PCR

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Russell Durrett

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Jan 14, 2011, 8:13:50 PM1/14/11
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Hey guys,

If you remember a couple of weeks ago someone posted a link from 2002
about a PCR machine built using a light bulb as the heating element. I
thought it was a great idea, so I built one myself.

I added a fan to cool faster, controlled the whole thing with an
arduino and a thermistor to monitor temperature. There's a ton of room
for improvement, so I encourage you to build a better one! My
schematics and arduino code are available at my site to help get you
started. Check it out here:

www.russelldurrett.com/lightbulbpcr.html

Russell Whitaker

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Jan 14, 2011, 8:29:19 PM1/14/11
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What timing: I'm sitting here right now waiting for this event to start:
http://techshop.ws/Upcoming_Events.html?&action=detail&id=107

I'm already a member of TechShop SF, so I have everything I need
to make one of these myself, and I'm going to do just that;
thanks (the other) Russell!

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http://orthonormalruss.blogspot.com/

Andrew Hessel

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Jan 14, 2011, 10:38:24 PM1/14/11
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I think this is terrific!  Thank you for sharing this...

Nathan McCorkle

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Jan 14, 2011, 11:19:09 PM1/14/11
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Looks good! Where do you put the microtube(s)? I would also add a
diode across each of the relay switch leads to protect your
microcontroller:
http://interactive.usc.edu/blog/?p=2330

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Ruediger Trojok

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Jan 15, 2011, 8:03:41 PM1/15/11
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really excellent! I hope it will work well.
To me it looks like you put the tubes in the drilled wholes in the can
surrounding the light bulb.
It may happen though, that the tubes will be too far outside of the
heat.
This could affect efficiency and may lead to condensed water at the
lid.
Why not put the tubes completely inside the can?

On 15 Jan., 05:19, Nathan McCorkle <nmz...@gmail.com> wrote:
> Looks good! Where do you put the microtube(s)? I would also add a
> diode across each of the relay switch leads to protect your
> microcontroller:http://interactive.usc.edu/blog/?p=2330
>
>
>
> On Fri, Jan 14, 2011 at 8:13 PM, Russell Durrett <rover...@gmail.com> wrote:
> > Hey guys,
>
> > If you remember a couple of weeks ago someone posted a link from 2002
> > about a PCR machine built using a light bulb as the heating element. I
> > thought it was a great idea, so I built one myself.
>
> > I added a fan to cool faster, controlled the whole thing with an
> > arduino and a thermistor to monitor temperature. There's a ton of room
> > for improvement, so I encourage you to build a better one! My
> > schematics and arduino code are available at my site to help get you
> > started. Check it out here:
>
> >www.russelldurrett.com/lightbulbpcr.html
>
> > --
> > You received this message because you are subscribed to the Google Groups "DIYbio" group.
> > To post to this group, send email to diy...@googlegroups.com.
> > To unsubscribe from this group, send email to diybio+un...@googlegroups.com.
> > For more options, visit this group athttp://groups.google.com/group/diybio?hl=en.

John Griessen

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Jan 15, 2011, 8:45:01 PM1/15/11
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On 01/15/2011 07:03 PM, Ruediger Trojok wrote:
> Why not put the tubes completely inside the can?

That design may be getting air flow by gravity convection and
the holes are where the flow is, and so the best even temperature...
So, if inside the can, at least near the holes for flow.

No one says you can't put insulation around the can and the tubes either.

What if you had multiple smaller incandescent bulbs and air vents
as often as bulbs, but alternating in position as you go vertically?
Then you would get a swift chimney effect from the combined heat
and alignment of bulbs vertically for even heat and good transfer and fairly rapid cooling down also,
and you could stop direct radiant heat gain, since that can go up too fast
with a little variation in position...

Such a machine would have a small desktop foot print since
it is chimney shaped :-)

John

Russell Durrett

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Jan 16, 2011, 3:16:31 PM1/16/11
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I added a video to the site explaining more about the machine.

Nathan - the tubes are inserted into the holes on the top segment,
holding them near the light bulb.

JonathanCline

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Jan 20, 2011, 4:19:45 AM1/20/11
to DIYbio, jcline
On Jan 16, 12:16 pm, Russell Durrett <rover...@gmail.com> wrote:
> I added a video to the site explaining more about the machine.
>
> Nathan - the tubes are inserted into the holes on the top segment,
> holding them near the light bulb.
>

This is an excellent writeup of the hardware project (schematic,
video showing hardware components, software posted) !
That's a great example of open source. I suggest adding a BOM
(bill of materials, i.e. a table with 4 columns listing: part
number,
description, quantity needed, and price), to make the project
fully complete.


I previously had a paper regarding light bulb PCR ; I believe a bit
old
(1980's?) and used an IBM PC 8088 or something to control the
heater.



## Jonathan Cline
## jcl...@ieee.org
## Mobile: +1-805-617-0223
########################


Cathal Garvey

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Jan 20, 2011, 5:40:32 PM1/20/11
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I agree, this whole project smacks of open-source, low-cost class. Awesome work, Russell!

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Cathal Garvey

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Jan 20, 2011, 5:41:48 PM1/20/11
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Oh, Brian and I are presenting a bunch of stuff at Newcastle Maker Faire 2011, including at least some paper presentations of great projects. If you have a project writeup that'd fit on a sheet of A4, we could throw a stack of them on the table?

Mackenzie Cowell

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Jan 27, 2011, 3:23:42 AM1/27/11
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GEL!  I want to see a gel!  Guess I should make one and try it!

And I suggest halogen lighting.  It just looks so much nicer :)

Very cool Russel!  I truly hope we can get the kinks worked out and standardize a $30 thermocycler (w/ arduino).

Mac
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