hi there

[Ian Wagers]

I not sure if this is the proper place to ask this, im new to biotech and I was considering dabbling in it some to see if I like it maybe try to start a small lab, let me know if you have any suggestions on were to get started. I do not have much knowledge in it.

[Koeng]

To start, I recommend just getting a Carolina transformation kit, do it, and see how you like it

http://www.carolina.com/transformation-dna-transfer-kits/glow-in-the-dark-transformation-kit/FAM_211086.pr?catId=&mCat=&sCat=&ssCat=&trail=SRCH:transformation:7002:10146&question=transformation

[Fred Kittelmann]

One reason for my interest in diy bio was to be able to do some of my own health care.  This is a far-off goal.  I'm 48 and healthy at the moment, but who knows what I might need decades from now, and what might be possible decades from now.  Access to health care is problematic at present, and I'm not optimistic about improvement in that regard.  Best to develop capacities and wherewithal now.  Diy might be the only option at some point.

I figured on taking many years to establish facilities where I live (Philadelphia) and get up to speed on modern methods.  (I've got a decent amount of book-learning, but haven't worked in a lab since the 90's.)  I did not expect a need for diy medicine to be right around the corner.  But here it is.  My mom has breast cancer.  She's insured, had successful surgery, and expects chemo in the near future.  And that may work out fine.  But what if it doesn't?  I wonder if there aren't things we can do to help her right now.  Her cancer is of the HER2 type.  Knowing that is useful for tailoring treatment.  Perhaps there's more helpful information about her case that could be learned, but medical practice doesn't include testing for such.  Does she have some mutation causing her to overexpress the HER2 protein?  Perhaps it would be useful to know what that is.  If things ever got real bleak, she'd be willing to try well-reasoned, innovative approaches not part of standard medical practice.  Is there anyone on this list who'd be willing to consult and/or conduct laboratory work toward such ends?  We'd be willing to compensate.

Thank you!
Fred Kittelmann
215-551-1490

[Mega [Andreas Sturm]]

Hi! I heared that injecting vitamin C could heal cancer in some cases. I am not sure I believe these rumors, but if all other treatments wouldn't have worked, I think I'd try it for myself. Worst case it don't work...

[Eugen Leitl]

No. Worst case you die. Endotoxins and sepsis are not just words.

[Cathal Garvey (Phone)]

Also, it's untrue. Linus Pauling is responsible for that big lie: somehow he got the notion that all human ailments stemmed from an insufficiency of Vitamin C, and used his nobel prize (physics) to spread the word.

Physicists: biology and medicine don't reduce well.

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Sent from my Android device with K-9 Mail. Please excuse my brevity.

[Mega [Andreas Sturm]]

Ok, sorry for the misinformation then..

[Stephanie]

Regarding gene mutations, there are DNA analysis services such as 23andme, where you can send in a saliva sample, and they process your DNA. They do not tell you what your mutations are, however. They just give you a simple ‘chances for contracting this or that’ overview. But, what’s really important for what you are after, is that they give you your raw DNA file, which you can then use in programs such as Prometheus to find out about your mutations. The group may know of other DNA analysis services that may be even better, as I am just an interested party... I hear for further gene mutation analysis Prometheus is the best, however, I would also be interested in hearing about other applications out there that could be even better....

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[Dan]

Pauling is the only person who has won two undivided Nobel Prizes. The Nobel Prize in Chemistry in1954 and the Peace Prize in 1962. 

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[Cathal Garvey (Phone)]

..which is cool for him, but doesn't qualify him to make poorly supported pseudoscience claims that can actually kill people if taken seriously.

[vrgopal]

Well said

V.Rajagopalan

Sent from my iPad

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