Synechococcus elongatus UTEX 2973

[Jonathan Cline]

These cyanobacteria in general are freshwater photoautotrophs. 
Might this new little guy be edible after washing?
   


Quote:

Efforts focusing on understanding cyanobacterial
systems are underway; however, one drawback compared to E. coli or yeast is that the growth rates of commonly
used cyanobacterial model strains are significantly slower, requiring extended timeframes (weeks to months) to
accomplish synthetic biology experiments that can be performed in E. coli or yeast in days.
[...]
Growth of Synechococcus UTEX 2973 was assessed under different
conditions and the shortest doubling time was 1.9 hrs in BG11
medium19 at 41uC under continuous 500 mmoles photons?m22?s21
white light with 3%CO2. This is a remarkably high growth rate under
autotrophic condition, the highest rate reported to date for a
cyanobacterial strain, and comparable to heterotrophic growth
rates of the yeast S. cerevisiae. The doubling time of Synechococcus
UTEX 2973 increased to 2.3 hrs at 38uC (Table 1).
[...]
Culture conditions that are optimal for the rapid growth of
Synechococcus UTEX 2973 (38–41uC, 3% CO2 and 500 mmole
photons?m22?s21 using BG11 media) are utilized in many laboratories,
and no special nutrients, such as vitamins, are required for the
growth of this cyanobacterial strain. This strain grows so rapidly, in
culture volumes ranging from 50 ml to 100 L, such as in a photobioreactor,
that contamination was not apparent even when growth
media were not sterilized and the systems were semi-open to the
outside. On solid BG11 plates, at 38uC and under 200 mmoles
photons?m22?s21 light and in ambient air, single colonies of
Synechococcus UTEX 2973 were visible within 2 days after plating
from a very dilute liquid culture, and these colonies were large
enough for passage by the third day.
[...]


End Quote

Ref:

Yu, Jingjie; Liberton, Michelle; Cliften, Paul F.; Head, Richard D.; Jacobs, Jon M.; Smith, Richard D.; Koppenaal, David W.; Brand,
Jerry J.; and Pakrasi, Himadri B., ,"Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using
light and CO2." Scientific Reports.5,. 8132. (2015).
http://digitalcommons.wustl.edu/open_access_pubs/4285

-- 

## Jonathan Cline
## jcl...@ieee.org
## Mobile: +1-805-617-0223
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[Nathan McCorkle]

Very cool!

Looks like there is a site that would sell it, but they're out of stock:
http://utex.org/products/algae-express-utex-2973-synechococcus-elongatus

interestingly that page says it was mutated from this strain which was
isolated in 1952:
http://utex.org/products/utex-0625

That page has a seemingly informative blurb, and mentions this is produced:
https://en.wikipedia.org/wiki/Metallothionein
which seems like a sort of chelator... so I'd guess at least that
these proteins would need cooked or denatured somehow, so they didn't
interfere with digestion if this was used as food.

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--
-Nathan

[BraveScience]

Hi,

I work with cyanos and I had chance to play with this bug.
They all smell pretty bad.

Synthetic biology in this strain isn't that straightforward. Cannot natural transform and conjugation takes ages. Like weeks.

But having colonies after two days is like working with yeast.

Fede

[Yuriy]

are you sure you want Blue-Green algae as food-stuff producer?

brief article: http://newsroom.uts.edu.au/news/2013/09/breakthrough-discovery-links-blue-green-algae-with-motor-neuron-disease

full text: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075376

option #2: http://www.ncbi.nlm.nih.gov/pubmed/15817793

Then again everyone seems to be advocating for Spirulina. 

[Alex D]

Spirulina also has a problem when other bacteria is present it produces very toxic exotoxins, it could be knocked out to keep the culture but who knows what else is produced. But it definitely would be easier to eliminate exotoxins in bacteria then in algae due to genome size.