Re: [DIYbio] pJE202 pVIB in liquid culture

[Matt Lawes]

If glowing is quorum sensing dependent .... there will be a higher local concentration of inducer with a colony than in liquid culture where the induced can more easily diffuse away.

>matt

Sent from my Verizon Wireless 4G LTE DROID

Josiah Zayner <josiah...@gmail.com> wrote:

To be more specific, when I grow it on a plate I see it fine. But when I grow it in liquid culture alongside the plate I don't see anything. Since the liquid culture is more dense I would expect to see it better? I know in the original paper they added a shit ton of stuff to the culture. Wondering if any of that is actually required? If there is a specific strain of E. coli that works better or worse? If quorum sensing can help or inhibit it(should help?)?

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[Sebastian Cocioba]

Gonna need to transform some pVib for some art students so Ill send
pics if it glows in liquid. Did you make sure to grow it at room temp?

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Josiah Zayner
Sent: ‎7/‎20/‎2014 2:49 PM
To: diy...@googlegroups.com
Subject: [DIYbio] Re: pJE202 pVIB in liquid culture
Cool. Let me know how it goes.

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