Visual ID of Tricoderma spp

[scoc...@gmail.com]

See attached picture, please.

I got this packet of powder from kelp4less.com which says its tricoderma soil amendment. I plated them on PDA media (old Carolina bottle) using a sterile swab dipped in the powder dry and sprinkled and/or swabbed on the surface. They were incubated upside down at room temp wrapped in tin foil for darkness on january 6th of this year and i just unwrapped them today.

My question is does anyone think what Im seeing here is tricoderma? Viridae? Reesei? Either way (no pun intended), I'm just curious if this is what I got. Also, know of any fungal barcoding primers and/or simple fungal DNA prep? I'm gonna try with Edwards buffer once I get primers in for amplifying out the hydrophobin coding sequence from trico reesei. Any thoughts?

I know visual ID is difficult and seldom recommended but as a hunch, think I got it?

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

[Dakota Hamill]

Looks like trich to me.  Check them out at 100x they have pretty unique conidia.  Plant spin kits work well for fungi, and though basidiomycetes worked 100 percent of the time for us, ascomycetes were harder.  

Spin columns take a long ass time and weren't consistent on molds as the spores are tough and old mycelia dry up behind a chitin wall.  We ended up having better success by taking a toothpick scoop of fresh, fresh is key, mycelia and putting in 100ul of water.  95C for 3 minutes.  5ul as template in 50ul run.  

ITS1F AND ITS4R are good.  I have tubes I can send out in the mail tomorrow if you'd like them. 

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[Xabier Vázquez Campos]

Here you can find all you need for the ID of Trichoderma isolates
http://www.isth.info/

A single PCR however, it's likely to be not enough to ID a Trichoderma sp. as they form a species cluster and the ITS is not sufficient. It will give you at least one of the about 14 subclades of species anyway. You may need to do EF or something else.