NYSM agar media?

[Paul Anderson]

What exactly is NYSM media? I just noticed that Boreal sells it, and
lists it as live. A google search is less than helpful...

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[J. S. John]

NYSM = Nutrient Yeast Extract Salt Medium

According to this article [1], it is a conventional lab growth medium.
They used it to culture bacteria.

You should have tried to google NYSM medium. Gives better results.

[1] http://www.formatex.info/microbiology2/349-358.pdf

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[Cathal Garvey]

Taken from that reference here's your recipe:

To 1L deoinised water, add:

5g Peptone

5g Glucose

3g Beef Extract (oxo-style soup cubes? Watch for added salt/oil content)

0.5g Yeast Extract

Some unspecified amounts of MgCl2, CaCl2, MnCl2.

The salts were referenced poorly. The paper said to add "10ml" of them, not mentioning anything about concentration/molarity of any of the salts.

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[Mac Cowell]

Via the beer brewing discussion in other thread, the salts cathal mentioned are often available at homebrew stores at good prices.

231.313.9062 // @100ideas // iPhoned

[Cathal Garvey]

Cool, I think I can use some MnCl, I'll check out brouwland for it.. :)

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On 25 May 2011 04:55, "Mac Cowell" <m...@diybio.org> wrote:

Via the beer brewing discussion in other thread, the salts cathal mentioned are often available at homebrew stores at good prices.

231.313.9062 // @100ideas // iPhoned

On May 24, 2011, at 11:44 AM, Cathal Garvey <cathal...@gmail.com> wrote:

> Taken from that reference here's your recipe:
>
> To 1L deoinised water, add:
> 5g Peptone

> 5g Gl...

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