Substituting Sodium Thiosulfate with EDTA for alkaline lysis
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benjamin liang
Aug 13, 2013, 11:23:52 PM8/13/13
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Hi guys,
I was wondering if it is possible to substitute Sodium thiosulfate for Disodium EDTA as a chelating agent for alkaline lysis of E coli cells.
I would also like to know if sodium acetate could be used in place of potassium acetate for the precipitation of genomic DNA and renaturing of plasmid DNA.
Thanks!
I was wondering if it is possible to substitute Sodium thiosulfate for Disodium EDTA as a chelating agent for alkaline lysis of E coli cells.
I would also like to know if sodium acetate could be used in place of potassium acetate for the precipitation of genomic DNA and renaturing of plasmid DNA.
Thanks!
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