expired lb broth powder -- usable?

[Daniel Packer]

From 2010 and 2011. Two different brands. Thoughts on using LB powder
after expiration? What could go wrong? Should I toss or try to use?

Thanks.

DP

[Cathal Garvey]

Lysogeny Broth is a pretty simple medium, I don't imagine there's much
that can go wrong. As long as it's not actually after spoiling, of
course. If it's been stored as a dry powder and nothing has grown in it,
it's probably ok.

For consistency though, don't compare results to "fresh" LB. There are a
lot of vitamins in the yeast extract in LB, for example, that may have
degraded. While the bacteria will probably grow without issue anyway,
growth rates or somesuch may be a little different.

So, I'd use "expired" LB without question for things like growing cells
for minipreps, or culturing microbes to keep stocks, but not for
comparing growth or gene expression patterns etc.

--
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[Mega]

As Cathal said,  for most purposes it should be no problem.

In former times food was conserved by dehydrating (Ok, still today). Without water bacteria can't digest it.

Vitamins may dissociate, but bacteria can produce their own vitamins.

[Nathan McCorkle]

On Jun 15, 2012 6:13 AM, "Mega" <masters...@gmail.com> wrote:
> Vitamins may dissociate, but bacteria can produce their own vitamins.

Only if they're autotrophs.