cheaper DIY protease inhibitors

[Andreas "Mega" Stuermer]

Hi everyone! 

Just wanted to see if someone has had ideas how to make your own protease inhibitor cocktail. PICs are pretty expensive, like >500$ to stabilize 500 mL of liquid protein extract. 

I remembered finding, for example, that the stain "trypan blue" that people use for mammalian cell culture is also used as a dye for jeans, so you can either buy 10 mL for 50$ from Thermofischer, or 1 kg for 50$ (didn't look at prices on alibaba) if you are willing to go through the development proccess. 

EDTA is a component of protease inhibitor cocktails and is quite cheap (roughly 150$ for a kilogram) , but I don't know yet about the other substances. 

Would be interesting if someone has looked into this

[Andreas "Mega" Stuermer]

https://bitesizebio.com/58195/protease-inhibitors-101/

https://info.gbiosciences.com/blog/how-to-select-the-right-protease-inhibitor

The most commonly used protease inhibitors apparentlly are:
AEBSF

APROTININ
EDTA
BESTATIN
APROTININ
E64
LEUPEPTIN
PMSF
PEPSTATIN A