Do I need to replenish Kanamycin like Ampicillin?

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Hans Wilms

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Aug 15, 2021, 8:33:47 AMAug 15
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Hi DIYbio group,

I'm growing E. coli (BL21(DE3)) in a bioreactor with a Kan resistant plasmid for protein expression. Do I need to replenish the Kanamycin every few hours during E coli growth like I do with Ampicillin, or am I good with just the starting amount (50mg/mL)?

If I do, could you recommend a protocol or starting replenishment rate?

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-Hans
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