interesting!

[Avery louie]

http://www.kickstarter.com/projects/1368829405/bacteriography-photographs-grown-in-bacteria?ref=NewsApr0413&utm_campaign=Apr04&utm_medium=email&utm_source=newsletter

Anyone know what it is?

It looks like some kind of nutrient/growth promoter was plated down and the bactos grow on it, or there is antibiotic elsewhere.

--A

[Nathan McCorkle]

Looks like contact lithography using UV to kill the bacteria in exposed regions. He's using GFP E.coli and S. Marcescens.

http://www.npr.org/blogs/pictureshow/2012/10/23/163391677/beautiful-bacteria-how-to-make-art-from-e-coli

"

^{"I take a petri dish with nutrient agar (the Jell-O-like stuff that the bacteria eat) and cover the entire surface with an even coat of bacteria. I then take a special photographic negative of the image that I want to 'print' and place it over the petri dish.}

^{"Next, I send radiation through the negative and onto the petri dish. As the radiation hits the negative, some of it is blocked and some of it passes through and hits the petri dish below. Where the radiation goes through the bacteria die, where it is blocked the bacteria grow. Take for example the Einstein image. The little red dots are where the negative blocked the radiation and the bacteria grew. The white space is where the radiation came through and sterilized the bacteria.}

^{"After exposing the bacteria latent petri dish to radiation, I allow it to grow for several days. After I feel that the image has finished 'developing' I irradiate the entire thing so the bacteria will stop reproducing. This 'fixes' the image and keeps the bacteria from spreading all over the place. Finally I use acrylic and resin to preserve and protect the bacteria."}

"

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[Avery louie]

Neat.  Too bad its not e. colloroid level neat.

--a

[Patrik D'haeseleer]

Yeah - very nice work. I've been working on something similar at BioCurious as well. In fact, I put in a question to the "Ask a biosafety expert" a while back, on whether sterilizing with UV and then embedding in clear resin is sufficient to be able to hand out a bacteria-laden plate to the general public.

Reading some of the news articles about his work, it sounds like he's using "radiation" rather than UV light. Some of the stories seem quite explicit about making that distinction, but it wouldn't be the first time a reporter got a detail like this wrong. Maybe an Xray source, with a photo negative made from an etched copper clad board?

Patrik

[Cathal Garvey (Phone)]

UV is technically radiation, perhaps he's sexing it up?

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[Nathan McCorkle]

I've actually done a really crappy version of this with a UV lamp, plastic wrap around an open petri dish, smeared with sunscreen on half of it. Repeated this with a few different SPFs, works markedly.

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