Please don't. There are already so many NPOs for DIY/community bio labs in the NYC area that unless you have a steady stream of curious minds coming and going your space will be just another struggling entity. Lets not forget the incubators.
When genspace realized that the a class based model brings in more revenue than a basic membership model they ran classes with little breaks. Every time you'd come in to further your goal, you wouldn't be surprised to find a class in session. It is frustrating to pay into a system like that and hear "no we cannot order that for you because of high trafic of novices and laymen." That was a couple of years ago. Their management changed hands but I don't know what restructuring that led to. Genspace could afford to do that. It has a decade old name recognition. Genspace itself sometimes reaches out into the city to teach a class/workshop there.
You could see if an existing community/DIYbio lab could use some help. Raising capital is always a topic no one wants to touch but everyone needs. Make yourself important. Become a part of an existing team. raise capital and negotiate to be be voted into an existing community/DIYbio lab.
Out of curiosity, where do you think of opening this space? my commute takes me an hour to a current community lab.