miniature flourescence microscope

[Michael Shamberger]

Hi,

I was thinking to build one of these.  

http://www.nature.com/nmeth/journal/v8/n10/fig_tab/nmeth.1694_F1.html

They are being made by a company called inscopix but are not available to the public.  They attach them to rat brains and watch their neurons fire as the rats run around.  

I am not planning to attach it to any brains but thought it could be useful if an array of these could be deployed in an automated microscope.

cmos sensor - raspberry pi

dichrioc mirror, emisson filter, excitation filter - ebay then cut down to size

filter cube and case - start with 3d printed but then see if there is some standard part available

led - ebay

I am not sure what the specifications of the achromatic lens should be though.  If anyone has ideas please let me know.

Mike

[Nathan McCorkle]

Why didn't you just update the topic you already started on this?

If you're only doing single-color, chromatic aberration isn't a concern at all.

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-Nathan

[Michael Shamberger]

>> Why didn't you just update the topic you already started on this?

Just thought it was a different idea.

I was thinking about one color but then I saw the RGB Leds.   https://www.inventables.com/technologies/miniature-smart-led-blinkm-minm

The nature papers has a pretty good description of the types of lenses they used:

"A microscale drum lens in the nVista HD collects the LED light and passes it through an excitation filter. The light is then deflected by a dichroic mirror and enters the imaging pathway of the device. A gradient-refractive-index-objective lens then focuses the light onto the subject sample. The fluorescent dye in the sample reacts to the light and the resulting illumination is captured by the objective lens, deflected off the dichroic mirror, passed through an emission filter and finally an achromatic doublet lens sends the image to the CMOS sensor."

Here are the lenses they describe:

drum lense - They used a drum lense instead of a collimating lense in front of the led.  What is the benefit of this or does it serve the same purpose?  I ordered a glass collimating lense that would normally be used in a laser pointer.

achromatic doublet lens -  I ordered this: http://www.ebay.com/itm/221484989690?ssPageName=STRK:MEWNX:IT&_trksid=p3984.m1439.l2649.  They are a little big but that is ok for the prototype and they are $4 each.

gradient-refractive-index-objective - Need to learn more about "GRIN" lenses.

Filters

Emission filter- Still looking.   The ones I have found are 3mm thick so I am not sure if those will work in the mini filter box

Excitation filter- Still looking

Dichroic mirror-  Found a small one on ebay that would be good for single color FITC/eGFP flourescense.  That is ok to start with.

[Nathan McCorkle]

The grin lenses might be based on the LED brightness across its beam waist (cone of light).  If that isn't nice and smooth and mostly flat, the subjects won't be evenly illuminated and image quality would suffer. It could be correcting other aberration too, but I doubt it would be chromatic unless they specifically mentioned multiple colors (or if it is common to use multiple colors these, which I don't know).

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