Microfluidics of single bacteria?

[Ana Santos]

Hi DIYbio community!

I'm wondering if anyone is aware of a high-throughput microfluidic chip for long term time-lapse microscopy of individual bacteria.

Most chips I come across are intended to monitor the growth of bacterial communities. I want to monitor individual bacteria.

Thanks in advance for your assistance.

Best,

Ana

[Otto Heringer]

Hi Ana,

I know someone who did this to watch some Xylella fastidiosa swimming.

This bacteria have a veeeery slow life cycle - and have a low motility as well. So this guy acomplished doing this for long periods of time, in the order of days. He built a single channel mimicking the phloem of trees - this bacteria is phytopathogenic.

If your are interested I could put you in contact.

But first, what you are planning to do? Bacteria swimming competition? (kidding)

Share a bit more what's the big plan!

Best,

Otto

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[Jonathan BISSON]

Ana,

https://www.youtube.com/watch?v=y2rNLRv2t5c

They seem to do cell trapping here.


J.

[John Griessen]

On 07/18/2017 11:33 AM, Ana Santos wrote:
> wondering if anyone is aware of a high-throughput microfluidic chip for long term time-lapse microscopy of individual bacteria.

So, by high throughput, do you mean individuals are tracked and snapshotted, and 1000 of them are in a pipeline?

Sounds difficult to keep them fed. Like a job for an AI cell herder...keeping them separated by air bubbles, stirred, aerated,
food injected, illuminated and snapshot every time interval.

Do you have a budget to develop it if not found for sale?

[Abizar Lakdawalla]

The joint genome institute does single bacterial cell isolation and growth with microchambers ... They are based in Walnut Creek CA and have very good community outreach programs.

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