Can you freeze cellulolytic enzymes?

[Sebastian S. Cocioba]

I cant find a clear answer. I know cellulase and or macerozyme can be stored in the fridge at 4c for 24hr as an aqueous solution but what happens post freeze-thaw at -20c? Its not very thermostable but does that effect it at low temps? I know freeze-thaw cycles brutalize most enzymes but what if its just one cycle. Like a keep frozen till use method. Curious if anyone has experience with that since these tend to be pricey and I would hate to lose a ton of activity thanks to one freeze-thaw cycle.

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

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[jlund256]

Enzymes are routinely stored in a 50% glycerol solution at -20C.  They do not freeze because of the glycerol.  Freeze / thaw would likely kill or seriously reduce enzyme activity.  All these things are enzyme specific.  Some enzymes don't like low temps or glycerol, but this is relatively rare.  If these enzymes are sold commercially, check if anyone sells them as a glycerol stock.

 

Jim Lund

 

[Cathal Garvey]

The use of glycerol is probably to prevent having to freeze/thaw enzymes
with each use; they can be used at -20 and immediately put back in
storage. So, I'd mimic this and use glycerol to keep enzymes happy.

Too much glycerol in the final reaction mix can mess with some enzymes
though, particularly DNA-modifying enzymes, so you may need to assess
how much you add and use the minimum needed to keep solutions liquid at
-20. I doubt this would have much bearing on cellulolytic enzymes, but
you never know!

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