DIY tube holder for vortex mixers
CodonAUG
mixers. I needed something to hold microcentrifuge tubes for me
because I didn't want to stand there for 10 minutes with my arms
getting uncomfortably shaken.
Check it out if you are interested.
http://cheapassscience.wordpress.com/2012/01/20/diy-tube-holder-for-vortex-mixers/
Chris
David McWilliam
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CodonAUG
about it? Maybe I can offer some ideas.
On Jan 20, 2:26 am, David McWilliam <dmcwill...@gmail.com> wrote:
> Great post. I've been looking for something like this for macerating plant
> material in bead beater tubes prior to DNA extraction. I've just bought a
> dremelfuge which arrived today and am thinking of ways to attach it to a
> vortexer. The horizontal tube position would help maximise the bead
> vortexing although it may need something to stop the tube caps from
> popping. Maybe a vortexer modification of the dremelfuge - Cathal?
> DMcW
>
>
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>
>
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> > I wrote up a blog post about how to make a tube holder for vortex
> > mixers. I needed something to hold microcentrifuge tubes for me
> > because I didn't want to stand there for 10 minutes with my arms
> > getting uncomfortably shaken.
> > Check it out if you are interested.
>
David McWilliam
CodonAUG
about? I see that in the picture the tubes are horizontal, you could
just cut slots in the foam so the tubes are horizontal rather than
vertical. This would be easier than mounting an inverse dremelfuge to
a vortexer.
On Feb 1, 3:49 am, David McWilliam <dmcwill...@gmail.com> wrote:
> I want to extract DNA from fresh/dried plant material. One technique is to
> macerate the tissue by placing it in a tube with beads and mounting it on a
> vortexer. There are commercial kits (http://www.geneworks.com.au/Product/ProductDetail.aspx?p=32&pid=9582) which
> dia) would work as well. My thoughts were to get a piece of 1.5" plastic
> tube to fit over the vortexer hub and cut slots in the top to correspond
> with the radial arms of the dremelfuge. The tubes may need restraint to
> avoid being shaken out (maybe a plastic wedge between the cap and the hub).
> I haven't tried any of these ideas yet - still looking for a suitable piece
> of plastic tube.
> DMcW
>
Cathal Garvey
dremelfuge as/on a vortex.
It's easy to shove an offset bearing into a dremelfuge; redesigning it
for that wouldn't be a great problem in OpenSCAD. The problem arises
when you spin it by whatever shaft goes through the bearing; you still
need to hold the dremelfuge main body from rotating with the shaft, and
the way you would do so would vary from drill/driver to drill/driver.
If you have a pre-existing vortex model (where there's just a hub that
oscillates without rotating) of course, it should be possible to
engineer the Dremelfuge simply to fit on top without too much trouble.
That's just a matter of replacing the dremel-adapted hub into a
from-beneath mount matching the vortex's fitting.
So, in brief; vortex, easy (drill + offset skate bearing + mount, I use
a DIY'd one all the time). Unattended vortex, varies by design, haven't
bothered making one yet. Dremelfuge-that-fits-to-existing-vortex, Easy,
but I'd have to see pictures and precise measurements for the vortex mount.
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Nathan McCorkle
could compete with this 20 kg monster homogenizer:
http://www.mpbio.com/product_info.php?family_key=116004500
now this thing is heavy which means it can shake the hell out of the
tubes without falling over, this means tissue will homogenize very
quickly
this doesn't mean a normal vortexer won't work, but how long will it
take? will heat/degradation of cell components start to be an issue
because its taking long?
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
Cathal Garvey
vortexing with small metal beads that impact-crush everything inside.
Nathan McCorkle
Yeah I realized that
Sent from my mobile Android device, please excuse any typographical errors.
Cathal Garvey
Well, beyond what bead-maceration is, I can't offer opinions. I've never
used it, so I don't know how effective it will be. Depends on the
substrate I guess; plant leaf matter would probably respond really well,
for example, but meat probably not so much.
On 02/02/12 13:14, Nathan McCorkle wrote:
> Yeah I realized that
Patrik
developing a 3D printed head for Zymo's portable Xpedition Sample
Processor:
http://www.zymoresearch.com/devices-instruments/cell-disruptors/xpedition-sample-processor
http://vort.org/2011/09/30/spoon-bench-field-dna-processing-gadget-review/
It's essentially a modified Craftsman Hammerhead Auto Hammer, which
you can buy for $72 from Sears. But they're selling them for $992 with
a special adapter head to shake a 2ml tube with beads. So if you can
print your own adapter head, you can just buy the Hammerhead and save
yourself $900...
Cathal Garvey
tube holder in an hour's printing. In fact, I'm fecking certain I could;
I'd make one in two parts that interlock beneath the tube and form a
shaft at the other end that is gripped by the chuck; should be pretty
secure once fitted, and will securely grip the tube lid together.
Of course, you could also make a whole rack for holding tubes, but that
*would* be slightly more complicated to design without having it fall
apart under extreme hammer-action-ating.
Patrik
visited his lab last week - so you may want to hold off on that a bit
so as not to steal his thunder ;-)
I told him he should submit it to the Tekla 3D printing DIYbio design
competition, so I'm sure we'll be seeing it soon...
On Feb 3, 7:03 am, Cathal Garvey <cathalgar...@gmail.com> wrote:
> Oh, that's cheeky. I'm pretty sure I could knock out a chuck-fitting
> tube holder in an hour's printing. In fact, I'm fecking certain I could;
> I'd make one in two parts that interlock beneath the tube and form a
> shaft at the other end that is gripped by the chuck; should be pretty
> secure once fitted, and will securely grip the tube lid together.
>
> Of course, you could also make a whole rack for holding tubes, but that
> *would* be slightly more complicated to design without having it fall
> apart under extreme hammer-action-ating.
>
> On 03/02/12 07:58, Patrik wrote:
>
>
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>
>
>
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> > If bead beating is what you need, I know Russell Neches at UC Davis is
> > developing a 3D printed head for Zymo's portable Xpedition Sample
> > Processor:
>
> >http://vort.org/2011/09/30/spoon-bench-field-dna-processing-gadget-re...
Cathal Garvey
I'd only have been doing it for the rage and the lulz. My prototypes
tend to be dirty and barely functional. In contrast, Russell has a firm
history of awesome prototypes behind him. ;)
Nathan McCorkle
> If bead beating is what you need, I know Russell Neches at UC Davis is
> developing a 3D printed head for Zymo's portable Xpedition Sample
> Processor:
>
> http://www.zymoresearch.com/devices-instruments/cell-disruptors/xpedition-sample-processor
> http://vort.org/2011/09/30/spoon-bench-field-dna-processing-gadget-review/
>
> It's essentially a modified Craftsman Hammerhead Auto Hammer, which
> you can buy for $72 from Sears. But they're selling them for $992 with
> a special adapter head to shake a 2ml tube with beads. So if you can
> print your own adapter head, you can just buy the Hammerhead and save
> yourself $900...
>
I like this! The auto-hammer is currently $60 with free in-store
pickup... whether I can find an online coupon or not, I think I'm
going to pick one up. It happens I need to go to Sears to exchange
some tools anyhow... :D
the last time I tried to extract RNA from tissue, I was frustrated
with LN2 and mortar/pestle, though it was my first time I trying it. I
didn't get the cDNA I was expecting, and have since shelved the
project. Getting this to work would really encourage me to pick it up
again!
Nathan McCorkle
found the autohammer is on sale until tomorrow (9-Feb) for $44.99, so
after tax it came to about $49.
Brought it home and charged the battery, then whacked a soda can with
it, then brought it to my desk and whipped out the screw drivers to
take it apart!
Here are videos and photos of the guts! I think the only modification
I'd make to the hammer 'inside' is taking the spring from the
hammering tip and reversing it, such that instead of being in
'idle'/non-hammering mode normally, it would be in hammer mode
normally.
http://www.youtube.com/watch?v=ETghclmFTxE
https://picasaweb.google.com/109403794341975968814/AutohammerTakeapart?authuser=0&authkey=Gv1sRgCMWP2qe-yPao9QE&feat=directlink
John Griessen
> craftsman.com autohammer
I think the only modification
> I'd make to the hammer 'inside' is taking the spring from the
> hammering tip and reversing it, such that instead of being in
> 'idle'/non-hammering mode normally, it would be in hammer mode
> normally.
Using it for "with a special adapter head to shake a 2ml tube with beads."
makes me think of paramagnetic stainless steel beads... If you positioned
a vial with its closed end against a nonmagnetic anvil, surrounded it
with two solenoid windings, and alternated pulsing those windings, the beads
would move away from the end against the anvil and slam back. The whole thing
could be a cylinder 3cm wide and 5 cm tall with a DC supply cord leading away
to a 12V wall wart, or a USB port or powered ethernet port.
Nathan McCorkle
The only place I know to get paramagnetic beads is from bangslabs.com, not sure if they sell them unmodified (most of their stuff has surface modifications to allow attachment of other molecules)
I wonder if other companies or folks have tried just using alternating magnetic fields like John is recommending
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