Oh good angle! :)
> However, I wonder if it comes to questions of deregulating GM, if
> this system is really a helpful argument - as it is so stable.
> It definitively has a bigger survival chance in a wild ecosystem
> (lets say the guts after accidentally swallowing it) than a normal
> AMP marker, which becomes useless without selection pressure.
Very solid point; without antibiotics, antibiotic resistance is useless,
but colicin clusters are still useful if your main competitor is another
susceptible strain.
The most sensible answer is that it probably isn't a serious concern.
That's because Colicin V is very narrow spectrum; it *only* affects
E.coli, and only if they bear a set of 2/3 targeting surface proteins,
too. It won't help a strain like K12 survive against any other bacteria
that reside in the gut, so K12 won't survive.
So what about other E.coli that might adopt the plasmid? That's more
interesting, because the plasmid might be "loaded" with a payload that
might be not-so-good in the intestines. I'll grant you, although I don't
see it as likely, it's worth considering.
To resolve this, who thinks it *is* worth including a suicide gene on
the plasmid? It could be tet-mediated, so if, by some fluke or
misfortune, you ended up in hospital with a bacterium in your intestines
producing kryptonite, you could suggest a therapy they'll actually have
to-hand in the hospital which will contribute to the success of
eliminating the rogue DNA?
I should stress again that I don't worry about this, and neither should
anyone else. Colicin V is a peptide, and outside of a petri dish
communities of rival bacteria have plenty of ways of destroying
offensive peptides, or evading their effects. It's never even been
studied against biofilm-encased E.coli, which are probably more the norm
in gut flora anyway. This is all speculation, but speculation is fun and
also bears considering for PR and "precautionary" reasons.
On 29/01/14 22:38, Rüdiger Trojok wrote:
> Hey all,
> the bacteriocin functionality is pretty neat. I have suggested a similar
> system in my
> diploma thesis on a bacterial contraception, as it allows to give your
> modified strain
> an evolutionary fitness bonus. the interesting part is particularly, that it
> is a wide spread functionality
> in nature, appearing in various forms and concepts. the colicin V system is,
> as cathal says,
> in the EU considered as selfcloning. However, I wonder if it comes to
> questions
> of deregulating GM, if this system is really a helpful argument - as it is
> so stable.
> It definitively has a bigger survival chance in a wild ecosystem (lets say
> the guts after accidentally swallowing it)
> than a normal AMP marker, which becomes useless without selection pressure.
> Here, the question how common the concept in natural bacterial ecosystems is
> and if it really
> is an advantage compared to wild type bacteria or not.
> Any info on that? Any already made decisions by regulators on it available?
> Best,
> Rüdiger
>
> -----Ursprüngliche Nachricht-----
> Von: Nathan McCorkle [mailto:
nmz...@gmail.com]
> Gesendet: Mittwoch, 29. Januar 2014 22:55
> An:
diyb...@diybio.eu;
pdxhack...@googlegroups.com; diybio;
>
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> Betreff: Re: [diybio-eu] Re: IndieBB Crowdfunding Campaign: Help me make a
> great beginner's kit for DIYbio/synbio!
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