IdeaLab this Saturday!

[Nina DiPrimio]

Hello all-

Last IdeaLab we overviewed the flow of how to find and analyze a biosynthesis pathway using rose scent (geraniol) as an example.  I would love to discuss this pathway again in more detail as I really think it is something we should pursue for metabolic engineering. However, it would be great if someone from the group would be interested in leading a more in depth discussion on BioCyc/MetaCyc and the details of cloning of a gene from one organism to another (using the geraniol biosynthesis pathway as an example of course).  Let me know if you would like to take this on, and you can present it at a future meeting! Don't worry - if there aren't any takers, I will work through it :) But think it will be a great exercise for a future metabolic engineer!

This Saturday I would like to discuss the attached paper on gene expression.

Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.

Precise and reliable gene expression via standard transcription and translation initiation elements.
Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D.

Source

 

Nina DiPrimio, PhD
Anderson Lab
Department of Bioengineering
UC Berkeley

Lab Phone- 510-664-4200