iGEM Team Idea Help

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Feb 8, 2014, 11:05:55 PM2/8/14
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Hi everyone,
I'm leading an iGEM (HS) team.  We have decided to design e.coli that creates an insect AFP and creates a biofilm as it's default state.  It will be controlled by a kill switch.  We have quite a bit figured out, but I have a few questions that I was wondering if anyone could help out with.
1. Are there any failsafe ways of creating a biofilm in e. coli?  It seems there are many methods, but that few people have replicated them in genetic circuits.
2. What are the types of marker proteins that tag larger proteins to be carried outside of the cell?
Any help is appreciated. 

Kay Aull

Feb 9, 2014, 3:30:43 AM2/9/14
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I'm not an expert, but for #2 I would look into the Salmonella type III secretion system, e.g. http://www.ncbi.nlm.nih.gov/pubmed/19756048 (done by a SynBERC lab and presumably has iGEM-suitable parts).

Also, team Chile 2012 tried using OmpR (E. coli membrane protein) fusions, but didn't get very far, partly because they were using spider silk protein (a gigantic PITA to clone with).

- Kay

Joey Boots

Feb 9, 2014, 3:00:39 PM2/9/14
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Thanks, I'll definitely look into this.

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