Hey All,
Remember lightbulb pcr? Its
not the best way of doing pcr, but it is cheap and the parts are easy to get. One issue I had with referring people to the build or the idea of a lightbulb pcr was a lack of working builds.
In an effort to finish the project started at BOSSLAB, I brought the hardware home with me. I changed the sensor to a nice (and cheap) i2c sensor, and wrote some code.
Using the 2x PCR mix generously donated by
syd labs, I amplified some test DNA on the machine and ran it on one of my
homebrew gel boxes (dox coming soon). It worked!
Photo here with annotations of what lane is what.