Sars-Cov-2 was Lab Made Under Project DEFUSE

[D. Schlenk]

https://igorchudov.substack.com/p/sars-cov-2-was-lab-made-under-project?s=w


Sars-Cov-2 was Lab Made Under Project DEFUSE

Sars-Cov-2 is a Result of Years of Documented Scientific
Work


Igor Chudov

May 22, 2022


This long article will explain how Sars-Cov-2, the virus
that causes COVID-19, was created as a result of
intentional laboratory work. It will also show that the
blueprint for Sars-Cov-2 was described in the "Project
DEFUSE" proposal by Peter Daszak, which was preceded
by years of relevant lab work and virus manipulation.

I intend this to be a comprehensive "popular science" style
article, that has references and abundant links but is
generally understandable for regular science-minded
people, like journalism or computer science majors.

This article does not introduce any new ideas and is instead
meant to be a summary of accumulated knowledge about
"lab origin".

I do not set out here to investigate or debunk the coverup
efforts to hide the lab origin of Sars-Cov-2. These coverup
efforts involve scrubbing data, as well as promoting a so-
called "zoonosis theory", which is pushed by the NIH,
EcoHealth Alliance, and its funders and acolytes. I will
simply lay out laboratory-designed features of Sars-Cov-2
and will highlight past research where such features were
first discussed prior to 2020. This article is non-judgmental
and sticks to facts.

My hope is that reading this article will make you want to
send it to your smart friend, who will be able to understand
it and will become convinced that "lab origin" is not
"baseless misinformation".
Contents

Prashant's Jan 2020 article about strange HIV Gp120
and "gag" inserts in Sars-Cov-2 was withdrawn in haste
and under pressure during the weekend, which is unusual.

HIV-originated Gp120 inserts in Sars-Cov-2, described
by Prashant, are meaningful and instrumental to COVID-
19's infecting immune cells, similarly to HIV.

Furin Cleavage Site in Sars-Cov-2 does not exist in other
betacoronaviruses and is instrumental for pathogenicity.

Dr. Fauci is an expert on HIV and Gp120 glycoprotein
and is a co-author of many articles and a coinventor of
three Gp120 related patents, and no doubt understood what
Prashant's preprint meant, right away. Fauci and his
associates, together with the likely creator of
Sars-Cov-2 Peter Daszak, set out to cover up the origin
of Sars-Cov-2.

Peter Daszak's 2018 "Project DEFUSE" proposal describes
exactly what Sars-Cov-2 actually is.

Sars-Cov-2 was possibly cultured on a Moderna MSH3 cell
line containing a patented Moderna gene sequence
CTCCTCGGCGGGCACGTAG.



Hat Tips

While I followed the lab origin theory starting with the
Pradhan article, four people were instrumental in expanding
my knowledge of the "lab origin" of Covid-19. It is
JikkyLeaks (Twitter @JikkyLeaks), Arkmedic (substack),
@Daoyu15, and Charles Rixey (Twitter @CharlesRixey,
substack). Many ideas discussed were first brought up by
DRASTIC research. They, in addition to the respected
scientists I am citing, came up with almost all the ideas.

The only idea that I came up with, around February 2022,
is described in the subsection "HIV Inserts Infect Immune
Cells".



"Uncanny Similarity" to HIV Gp120 and gag Protein

In early 2020, as news of a novel virus killing people in
Wuhan emerged, the first genomic sequence of the virus
was published around January 20. On January 31, a
preprint by Prashant Pradhan was published, alleging that
Sars-Cov-2 "borrowed" certain genetic sequences from the
HIV virus:

Inexplicably, this paper was withdrawn only two days later,
on February 2, under murky circumstances. Mind you,
Prashant's article was published on Friday, Jan 31, 2020,
and was withdrawn on Sunday, Feb 2, 2020. So urgent and
important was the withdrawal, that important editors had to
be roused from their weekend activities to withdraw the
article.

A fact check was promptly posted on Feb 7, 2020, that
confidently declared HIV inserts a "baseless conspiracy
theory". Its author, Jessica McDonald, seems to be an
intelligent and informed person on a mission to cover
things up that her employer wants to cover up. That fact
check made a conclusion that was obviously false even in
January 2020 but is worth reading if you have time.

What are these inserts? Are they meaningless? Is the story
baseless? Who asked to write a fact check calling this story
"baseless"?



HIV Gp120 Inserts are Significant and Unusual

To remind my readers, Sars-Cov-2 is an RNA virus, that
has a strand of ribonucleic acid (RNA), enclosed within a
viral envelope, surrounded by "spikes". The spikes have
unique attachment points (receptor binding domains) that
attach to certain receptors on human cells. That attachment
allows the virus to open up the cellular membrane, inject
its RNA into the cells, and hijack the cell's ribosomes to
make new copies of the Sars-Cov-2 virus, as encoded by its
viral RNA. A good basic video of this is here:

What Pradhan found is that the viral RNA contains highly
unusual (for betacoronaviruses) sequences that appear to be
lifted from the HIV virus and inserted into the Sars-Cov-2
genome.

Fact-checkers stated that these small sequences are
meaningless. They are not.

Far from being useless genetic junk, the HIV inserts in
Sars-Cov-2 are very important functionally. Despite
appearing discontinuously, the proteins that they encode
come together in the final coronavirus spikes:

Interestingly, despite the inserts being discontinuous on
the primary amino acid sequence, 3D-modelling of the
2019-nCoV suggests that they converge to constitute the
receptor binding site. The finding of 4 unique inserts in
the 2019-nCoV, all of which have identity /similarity to
amino acid residues in key structural proteins of HIV-1
is unlikely to be fortuitous in nature.

Prashant provides a picture, that I further annotated:

These colored proteins that I circled, are on the outside of
the spike and are responsible for interaction with human
host cells. Prashant explains:

The insert 1 corresponds to the NTD (N-terminal domain)
and the inserts 2 and 3 correspond to the CTD (C-terminal
domain) of the S1 subunit in the 2019-nCoV spike
glycoprotein. The insert 4 is at the junction of the SD1
(sub domain 1) and SD2 (sub domain 2) of the S1 subunit
(Ou et al., 2017). We speculate, that these insertions
provide additional flexibility to the glycoprotein
binding site by forming a hydrophilic loop in the protein
structure that may facilitate or enhance virus-host
interactions.


A video illustration of HIV Gp120-specific binding sites is
seen in this video:

Twitter avatar for @JikkyleaksJikkyleaks (Fan account)
?? @Jikkyleaks

The 4 HIV inserts that Prashant Pradhan identified are on
receptor binding sites of the spike protein. The points most
likely to contact with a cell.

What are the odds that those inserts were located at those
sites in a natural process?

#pradhanwasright @c0v1d1984

April 13th 2022
406 Retweets808 Likes

We all have heard that Sars-Cov-2 enters host cells via a
so-called "ACE2" receptor, that is abundant in human
lungs. This is typical for many coronaviruses, and the
original SARS from 2003 also targeted ACE2. This is what
makes SARS-Cov-2 airborne.

That is not all it can do, however. SARS-Cov-2 has another
card up its sleeve.



Sars-Cov-2's HIV Inserts Infect Immune Cells, Like HIV
Does

SARS-Cov-2 also has a unique secondary mechanism: its
HIV inserts also allow SARS-Cov-2 to act just like HIV
and infect T cells of the immune system, contributing to
post-COVID immune depletion, without using ACE2
receptors at all. I wrote a long substack article about that,
but for the sake of completeness let me restate its major
points:

A Nature article, written by, among other people, Shi
Zheng-Li (the Chinese Batwoman from Wuhan Institute of
Virology), noted that many patients who had severe Sars-
Cov-2 had "lymphopenia", that is, depletion of the all-
important immune T-cells

The article shows Sars-Cov-2 virus is able to infect T-
cells via a unique (to coronaviruses) mechanism that
works like HIV, that also infects the same cells, causing
AIDS

They "show that the infection is spike-ACE2/TMPRSS2-
independent"

Infection of T-cells occurs via "LFA-1, the protein
[that] exclusively expresses in multiple leukocytes"

It turns out that HIV's gp120 protein, parts of which
Pradhan found in Sars-Cov-2, is the one that "Activates
LFA-1 on CD4 T-Lymphocytes and Increases Cell
Susceptibility to LFA-1-Targeting Leukotoxin"

Just like HIV, Sars-Cov-2 kills T cells: "infection of T
cells induced cell death that is likely in mitochondria
ROS-HIF-1a-dependent pathways"



Sars-Cov-2 Kills T-Cells, Just Like HIV

Is Sars-Cov-2 airborne HIV? Two days ago, an interesting
article came out: This article was not written by a bunch of
random scientists, but instead was written by people from
the Wuhan Institute of Virology, including the infamous
batwoman Shi Zheng-Li. Just keep this in mind. It was
originally submitted in Sep 2021 and revised in January
2022, so it d…


Read more
2 months ago · 331 likes · 265 comments · Igor Chudov

To summarize this part: HIV inserts in Sars-Cov-2 are
translated into instrumental proteins, that form highly
important "attachment points" on the spike protein. Those
attachment points, or "receptor binding domains", allow
attaching to several varieties of human cells, including
ACE2 expressing cells and immune T cells expressing
LFA-1. These HIV Gp120 inserts facilitate entry and
destruction of T cells, in a manner similar to HIV, using
LFA-1.



Furin Cleavage Site

Another, separate feature discussed in Pradhan's article, is
the "Furin Cleavage Site", or FCS. That site, also located
in the spike protein, is a sequence of proteins encoded as
"PRRAR", that cleaves (separates) the spike protein into
two parts when it encounters cellular enzyme furin. It
facilitates effective infection. FCS is not present in any
other sarbecoviruses.

Arkmedic explains:

And just to complete the quad-trick we need the last
insert sequence identified in Pradhan's paper which is
QTNS——PRRA. This is a really interesting sequence which
we will come to later, because is the furin cleavage
site. It's interesting because beta coronaviruses like
this don't have a furin cleavage site, this is the only
one. Surely this site couldn't have come from HIV-1?
Well, it's not from the GP120 protein like the other
three sequences, it's completely different and on a
different location of the virus which I'll show you soon
but for now let's run the BLASTp.

This allows for very high infectivity and pathogenicity of
Sars-Cov-2:

Unlike regular mutations (changes) usually replacing one
nucleotide for another, a complete and functional "insert"
is highly unlikely to be acquired by random viral evolution
in bats or other animals.

Thus, the PRRAR furin cleavage site insert is a result of
human engineering and is key to Sars-Cov-2 pathogenesis.

Arkmedic's picture provides a highlight of the FCS
(colored green on the picture below):

Here's a video of how Furin Cleavage Site works:
Anthony Fauci is an Expert on HIV and Gp120

Around the time Prashant's article appeared, a lot of secret
email activity (h/t @Jikkyleaks) was happening, discussing
how to hide the conclusions of Prashant's article.

To understand this situation, please note that the director
of NIAID, Dr. Anthony Fauci, is actually an expert on HIV
and Gp120. Fauci is a co-inventor of THREE patents
involving HIV's Gp120:

Dr. Fauci is also a co-author of many articles about HIV's
Gp120 glycoprotein:

Thus, around January 31, 2020, Fauci would have a full
and instant understanding of what HIV Gp120 inserts
meant for SARS-Cov-2. What did Dr. Fauci do? He
convened with his top associates Trevor Bedford and
Kristian Andersen, as well as Peter Daszak, and discussed
how they can hide these explosive findings.

Who is Peter Daszak and why is Peter talking to Fauci
about hiding evidence of Sars-Cov-2 being a lab product?

Well, Peter is the author of the proposal that gave us
SARS-Cov-2.



Peter Daszak and the DEFUSE Project

There is a document, called "Project DEFUSE: Defusing
the Threat of Bat-borne Coronaviruses".

This document, oddly enough, described a set of objectives
that aligns exactly with what was implemented as Sars-
Cov-2 and described above!

I annotated Peter's 2018 proposal with (numbers) so I can
explain how these sentences describe SARS-Cov-2
specifically and make it easy for you to see how they relate
to my article.

The search for a furin cleavage site represented Project
DEFUSE's desire to move on from 2003 Sars-1's less
effective trypsin cleavage site to something more
infectious, transmissible and pathogenic, which would be
a furin cleavage site. This concept was worked on by
scientists as early as 2006 when Kathryn Follis et al
attempted to replace the SARS-1 trypsin cleavage site
with a furin cleavage site. This shows that the DEFUSE
Project is based on preexisting laboratory discoveries.

An excellent explanation of interaction of Sars-Cov-2
with DC-SIGN and L-Sign is in a PLOS article "DC/L-SIGN
recognition of spike glycoprotein promotes SARS-CoV-2
trans-infection". Read that link if you have 10 minutes.

DC-SIGN is a dendritic cell lectin that binds to HIV's
Gp120 — and Gp120 is what was added to Sars-Cov-2.

Here Project DEFUSE describes testing their new virus
on human cells expressing above mentioned DC-SIGN.

Project DEFUSE anticipated problems infecting immune
cells (monocytes and macrophages) and thus added specific
Gp120 sequences allowing Sars-Cov-2 to infect those
specific cells via the above-mentioned LFA-1 mechanism.

Project DEFUSE tested the result in transgenic
(humanized) mice, having human ACE2 receptors in their
lungs. The mice had to be humanized: as we know, Sars-
Cov-2 was able to infect people, but not mice, at the
beginning of its history. (Side Note: Later on, another
famous virologist, Ralph Baric of UNC, worked on
allowing Sars-Cov-2 to infect regular — not transgenic —
mice. And guess what, somehow the later variants of Sars-
Cov2 (Omicron) do infect mice, a development that may or
may not be related to Baric's research. Baric was also on
the email thread with Fauci shown above.).

I believe that "commercial gene blocks" mentioned by
Project DEFUSE are HIV's Gp120 and Gag protein blocks.

Testing on humanized mice was not enough for Project
DEFUSE authors, and they finally tested their assembled
product on "HAE cells", which are "human airway
epithelial" cells just like the cells you have in your
lungs.

The last sentence (7): "test ... in Human Airway Epithelial
[cells] and in vivo pathogenesis", semantically, means lab
testing in Human Airway Epithelial cells, and then in vivo
testing in live HUMANS.


Stop for a second and read that again. What?

I am not sure if the authors really meant that sentence to
mean testing Sars-Cov-2 in humans, as English is not my
native language. But my understanding is that the plain
meaning of the above-quoted sentence is to refer to testing
in humans, describing in-vivo testing after in-vitro testing
on explicitly human cells.

Whatever "in … HAE cells and in vivo pathogenesis" meant to
the author, Sars-Cov-2 has plenty of in vivo pathogenesis
in humans, as the families of 6,299,692 dead COVID patients
would readily attest to.



Sars-Cov-2 has a Sequence from Moderna Patent 9,587,003
from 2018

Sars-Cov-2 has a nucleotide sequence CTCCTCGGCGGGCACGTAG.
Oddly enough, a reverse complement to this sequence exists
in a Moderna Patent 9,587,003, that describes certain human
cancer cell lines.
The probability of that sequence being in Sars-Cov-2 due to
random chance is estimated to be around one in
100,000,000,000. The most likely explanation for how that
sequence could end up in Sars-Cov-2 is that Sars-Cov-2
was cultured in Moderna-owned MSH3 cell culture at some
point.

For people who enjoy trying NIH's BLAST tool to hunt genes,
I wrote an article on how exactly to match the Sars-Cov-2
CTCCTCGGCGGGCACGTAG sequence to the Moderna patent sequence.
Try it out and have fun.



Disclaimers and Uncertainties

Let me mention a few things that my article leaves out:

I proved (based on the work of others) that Sars-Cov-2
was designed in a lab. I did not identify the lab, or the
person who did it (although Sars-Cov-2 originates from the
DEFUSE Project). I am very aware that Wuhan is the home of
Wuhan Institute of Virology. That said, WIV is not the
only possible source of Sars-Cov-2.

The DEFUSE Project proposal was rejected by DARPA.
However, EcoHealth Alliance was given money by DoD,
NIH, and other players such as the Bill and Melinda Gates
Foundation. Saying that DEFUSE was never implemented,
is incorrect. Project DEFUSE was implemented and the
result is Sars-Cov-2, which matches Project DEFUSE.
Here, federal money was given via DTRA (Defense Threat
Reduction Agency) for the exact purpose stated in Project
DEFUSE.

The sequence CTCCTCGGCGGGCACGTAG in Sars-Cov-2 does NOT
prove or even imply that Moderna was involved in creating
Sars-Cov-2. It merely proves lab origin.

We do NOT know whether Sars-Cov-2 was released
intentionally or accidentally. This article does NOT
attempt to identify any specific persons or reasons
involved in releasing it.

This article does not prove or even imply that Sars-Cov-
2 was implemented with the intent, from the beginning, to
be released and wreak havoc on humanity. I am leaving
this topic out.

Whether "Covid Vaccine" was created before Sars-Cov-2
was known officially, is a very important question, that
I do not want to discuss in this article, even though I
discussed it in the past.


Further Reading

For those curious to learn more, arkmedic provides
outstanding details on gp120 inserts, how they look, why
they are unusual and lab-made, and their functionality.
Please spare a half-hour of your time when you get a
moment to read the article below:

Arkmedic's blog

Absolute proof: The Gp-120 sequences prove beyond all
doubt that "COVID-19" was man-made

Following on from December's article "How to BLAST
your way to the truth about the origins of COVID-19"
where I explained how the four "inserts" described in
Pradhan's Feb 2020 paper showed definitively that the
SARS-CoV-2 genome had to have been man-made, there
has been considerable interest and a lot of push-back from
those whose reputations are on th…

Read more
a month ago · 104 likes · 80 comments · Dr Ah Kahn Syed


Famous economist Jeffrey Sachs wrote an article in PNAS
that is pointing out SOME lab-made features of SARS-
Cov-2 and is asking for an investigation.

However, unfortunately, Jeffrey Sachs is a brilliant
economist and not a virologist. His well-intentioned and
extremely well-written article left out HIV-related features,
and other facts, that are monumentally important, perhaps
due to article size restrictions. Jeffrey's article is what
prompted me to write this post — because I felt that he
unintentionally omitted the most crucial details.