The version of Samtools installed in your $PATH is not optimized for dDocent. Please install at least version 1.3.0

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Paige M.

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Aug 10, 2021, 4:03:44 PM8/10/21
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Hello, I followed the 2 suggestions made in another post about this same problem.  The post was started by valer...@gmail.com 2017 with a followup in 2020 for another person.

I am still getting this error.  My install is with miniconda3, not miniconda2 like the previous persons. Does that make any difference?  If so, what can I do?  If not, please let me know the options.  Oh, I did check my personal install of samtools and it is 1.11 which I updated to 1.13.


miniconda3/envs/ddocent_env/bin/dDocent: line 32: [: : integer expression expected
The version of Samtools installed in your $PATH is not optimized for dDocent.
Please install at least version 1.3.0

Paige M.

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Mar 30, 2023, 2:29:27 PM3/30/23
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Hello,
I gave up on this for a long time and just used another type of software to call SNPs.  Now - with a bit more experience and a new dataset I really wanted to try on dDocent pipeline- I solved the problem.  I did try the conda  update and new install of dDocent suggested again but that still didn't work.  Instead inside the ddocent conda environment I had created I simply had to run: conda install -c bioconda 'samtools>=1.16.1' 

Note that I definitely need to point to the version of samtools I wanted to get this to work.  Just an update or generic call to install samtools did not work.

Just adding this in case anyone else has a similar problem.  It is a simple fix, but I am someone who only uses computers for bioinformatics so simple problems can throw me easily!

Jon Puritz

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Apr 11, 2023, 10:53:00 AM4/11/23
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