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1–30 of 153
For debugging or troubleshooting, please include the following information:
The last output in
dDocent.runs
file
The entire
dDocent_main.LOG
The ouput of
ls -l
in your working directory
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Anthony Melton
3/13/24
error at line 1002: * 3 / 4: syntax error: operand expected (error token is "* 3 / 4")
Good day all, I am trying to run dDocent v2.9.4 on MacOS 14.4 and am running into this error: /Users/
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error at line 1002: * 3 / 4: syntax error: operand expected (error token is "* 3 / 4")
Good day all, I am trying to run dDocent v2.9.4 on MacOS 14.4 and am running into this error: /Users/
3/13/24
a.w...@uni-bayreuth.de
2
12/21/23
dDocent stops at creating alignment intervals
Let me add a detail: The dDocent_mail.log states the following: mawk: cannot open "mapped.1022.
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dDocent stops at creating alignment intervals
Let me add a detail: The dDocent_mail.log states the following: mawk: cannot open "mapped.1022.
12/21/23
safi...@gmail.com
10/18/23
Loci filtering based on SNPS counts (max 8 and min 3)
Deal All I want to filter loci based on SNPS, According to pipeline (perl rad_haplotyper.pl script),
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Loci filtering based on SNPS counts (max 8 and min 3)
Deal All I want to filter loci based on SNPS, According to pipeline (perl rad_haplotyper.pl script),
10/18/23
Melanie LaCava
,
Jon Puritz
8
10/11/23
massive 5.5TB uniq.k.2.c.2.seqs file - what's wrong?
That looks to have fixed the problem, thanks! On Friday, September 29, 2023 at 7:34:59 AM UTC-6 Jon
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massive 5.5TB uniq.k.2.c.2.seqs file - what's wrong?
That looks to have fixed the problem, thanks! On Friday, September 29, 2023 at 7:34:59 AM UTC-6 Jon
10/11/23
safi...@gmail.com
10/4/23
Extract Loci which has more than Specific of SNPS
I have vcf file with my ddrad data. I want to extract a handful of the loci for the skyline plot
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Extract Loci which has more than Specific of SNPS
I have vcf file with my ddrad data. I want to extract a handful of the loci for the skyline plot
10/4/23
Gracy Buckholtz
8/17/23
ls: cannot access 'mapped.*.bed': No such file or directory
Hi everyone, I am new to bioinformatics and job submission. I have been stuck trying to debug the
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ls: cannot access 'mapped.*.bed': No such file or directory
Hi everyone, I am new to bioinformatics and job submission. I have been stuck trying to debug the
8/17/23
Gracy Buckholtz
,
a.w...@uni-bayreuth.de
2
8/4/23
Installation Issues
Hi, I followed the conda install instructions for dDocent on a 'normal' Linux systemm (no HPC
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Installation Issues
Hi, I followed the conda install instructions for dDocent on a 'normal' Linux systemm (no HPC
8/4/23
Agostino Leone
6/22/23
extract SNP flanking region in fasta
Hi all, I realized that I need to extract the fasta sequences flanking some of my SNPs (the outliers)
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extract SNP flanking region in fasta
Hi all, I realized that I need to extract the fasta sequences flanking some of my SNPs (the outliers)
6/22/23
Paige M.
,
Jon Puritz
3
4/11/23
The version of Samtools installed in your $PATH is not optimized for dDocent. Please install at least version 1.3.0
Also, see note here: https://github.com/jpuritz/dDocent#if-you-are-getting-samtools-errors On Thu,
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The version of Samtools installed in your $PATH is not optimized for dDocent. Please install at least version 1.3.0
Also, see note here: https://github.com/jpuritz/dDocent#if-you-are-getting-samtools-errors On Thu,
4/11/23
Paige M.
,
Jon Puritz
2
4/11/23
Advice on using ddocent with wgs data
Hi Paige, It's not documented, but it is fully functional here (https://github.com/The-Eastern-
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Advice on using ddocent with wgs data
Hi Paige, It's not documented, but it is fully functional here (https://github.com/The-Eastern-
4/11/23
Santosh Kumar Rana
,
Paige M.
2
4/2/23
fail to run dDocent:: sed: can't read reference.fasta.original: No such file or directory
Hello Santosh, I'm a relatively new user of dDocent and I've been having a few problems with
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fail to run dDocent:: sed: can't read reference.fasta.original: No such file or directory
Hello Santosh, I'm a relatively new user of dDocent and I've been having a few problems with
4/2/23
a.w...@uni-bayreuth.de
3/17/23
Diagnostic SNPs from dDocent results
Hello, we have identified several hunderd of high-quality SNPs from MIG-Seq data of various fruit
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Diagnostic SNPs from dDocent results
Hello, we have identified several hunderd of high-quality SNPs from MIG-Seq data of various fruit
3/17/23
Alison Lam
,
Anthony Barley
2
2/1/23
Bioconda Installation Troubleshoot: Fail to run "conda create -n ddocent_env ddocent" in MacOS
Hmm, having a similar problem, does not look like recent posts on here have gotten many responses,
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Bioconda Installation Troubleshoot: Fail to run "conda create -n ddocent_env ddocent" in MacOS
Hmm, having a similar problem, does not look like recent posts on here have gotten many responses,
2/1/23
Tami Hildahl
12/1/22
Splitting data across multiple machines?
Hello - I have ~1400 samples to run across multiple (60) populations. Best estimate is 32 days of
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Splitting data across multiple machines?
Hello - I have ~1400 samples to run across multiple (60) populations. Best estimate is 32 days of
12/1/22
Russell P
3
6/1/22
negative Fis values in every population
The lab that generated the dataset said that " We noticed some regions got enormous number of
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negative Fis values in every population
The lab that generated the dataset said that " We noticed some regions got enormous number of
6/1/22
Russell P
2
2/24/22
phylip format from ddocent output
I've answered my own question. There's a handy python script that does the job: https://
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phylip format from ddocent output
I've answered my own question. There's a handy python script that does the job: https://
2/24/22
Xiaodong
9/2/21
reason for untrimmed PE reads for assembly
Dear Jon and others, I have a question about why dDOcent requires paried-end (PE) reads for de novo
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reason for untrimmed PE reads for assembly
Dear Jon and others, I have a question about why dDOcent requires paried-end (PE) reads for de novo
9/2/21
valer...@gmail.com
, …
Paige M.
11
8/10/21
Q&A
version of Samtools installed in your $PATH is not optimized for dDocent error when doing bioconda installation
Hello, I started a new conversation about this issue because this is older. Sorry if that wasn't
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Q&A
version of Samtools installed in your $PATH is not optimized for dDocent error when doing bioconda installation
Hello, I started a new conversation about this issue because this is older. Sorry if that wasn't
8/10/21
Russell P
8/1/21
mac vs. maf
In the SNP filtering tutorial, both mac and maf (minor allele count and minor allele frequency) are
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mac vs. maf
In the SNP filtering tutorial, both mac and maf (minor allele count and minor allele frequency) are
8/1/21
a.w...@uni-bayreuth.de
,
Jon Puritz
2
5/28/21
Quick start guide - clarification needed
For the subset, no trimming is needed to create the optimized assembly using the parameters you got
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Quick start guide - clarification needed
For the subset, no trimming is needed to create the optimized assembly using the parameters you got
5/28/21
Pearce Cooper
,
Jon Puritz
4
5/28/21
Best protocol for mix of SE and PE libraries
Hi Pearce, There's a lot to unpack here. First, that's an old version of dDocent, please have
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Best protocol for mix of SE and PE libraries
Hi Pearce, There's a lot to unpack here. First, that's an old version of dDocent, please have
5/28/21
a.w...@uni-bayreuth.de
,
Jon Puritz
5
5/28/21
dDocent stopped (line 1193: 1803 Killed)
They are in the dDocent.runs file Jon Puritz, PhD Assistant Professor Department of Biological
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dDocent stopped (line 1193: 1803 Killed)
They are in the dDocent.runs file Jon Puritz, PhD Assistant Professor Department of Biological
5/28/21
Russell P
,
Jon Puritz
3
5/6/21
time to run ReferenceOpt.sh
Hi Russel, This is really difficult for me to estimate for you. I would suggest though, not starting
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time to run ReferenceOpt.sh
Hi Russel, This is really difficult for me to estimate for you. I would suggest though, not starting
5/6/21
the...@puchner.net
,
Jon Puritz
4
3/8/21
Q&A
freebayes failed a third time
Thank you Jon! I will try both suggestions! Am Mittwoch, 3. März 2021 16:54:08 UTC+1 schrieb the...@
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Q&A
freebayes failed a third time
Thank you Jon! I will try both suggestions! Am Mittwoch, 3. März 2021 16:54:08 UTC+1 schrieb the...@
3/8/21
Joshua Thia
,
Jon Puritz
4
2/16/21
Exceeding memory limits for variant calling
Hi Josh, Again, this is going to depend on your samples, both number and coverage level. From you
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Exceeding memory limits for variant calling
Hi Josh, Again, this is going to depend on your samples, both number and coverage level. From you
2/16/21
safi...@gmail.com
2/12/21
Error: Contigs file must contain only contig header lines.
Dear All, Could you please suggest me how can I solve it? help please $ vcftools --vcf ZZ_ZW_BiSnps.
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Error: Contigs file must contain only contig header lines.
Dear All, Could you please suggest me how can I solve it? help please $ vcftools --vcf ZZ_ZW_BiSnps.
2/12/21
Jon Puritz
,
Alfons Weig
3
2/12/21
Re: Using dDocent for population genetic structure with diploid and polyploid populations, polyploids are asexual...
Any line with a freebayes command, so yes 489 and 507. For STRUCTURE, the best practice would be to
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Re: Using dDocent for population genetic structure with diploid and polyploid populations, polyploids are asexual...
Any line with a freebayes command, so yes 489 and 507. For STRUCTURE, the best practice would be to
2/12/21
M. Alicia Pascual R.
,
Jon Puritz
2
1/26/21
[E::hts_idx_push] Unsorted positions on sequence #...
My gut feeling is this has to due with weird characters introduced by NCBI. I would double check that
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[E::hts_idx_push] Unsorted positions on sequence #...
My gut feeling is this has to due with weird characters introduced by NCBI. I would double check that
1/26/21
Tereza Jezkova
,
Jon Puritz
2
1/26/21
Q&A
reference genome split to chromosomes
Hi Tereza, Yes, you should be able to simply concatenate them something like: cat *.fna >
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Q&A
reference genome split to chromosomes
Hi Tereza, Yes, you should be able to simply concatenate them something like: cat *.fna >
1/26/21
Jon Puritz
12/21/20
Q&A
Re: [dDocent User Group] dDocent naming convention
For debugging or troubleshooting, please include the following information: The last output in
unread,
Q&A
Re: [dDocent User Group] dDocent naming convention
For debugging or troubleshooting, please include the following information: The last output in
12/21/20