Affymetrix analysis call file not found

[Wendy Qiao]

Dear all,

I am running a hematopoietic lineage microarray in dChip. The platform
is Addymetix HU133 Plus2.0. I download CSV file (HG-U133_Plus_2
Annotations, CSV format) from Affymetrix library and made a gene info
file using the gene info file (HG-U95Av2) on dChip webite as an
example. I wonder if I have to have all of columns in HG-U95Av2, as I
cannot find "Gene Ontology.xls","Protein Domain.xls" "Chromosome" in
HG-U133_Plus_2 Annotations file. I presumed I have to have those
columns, and I used "Gene Ontology Biological Process", "Protein ID"
and "Chromosomal location" instead. Did I do it right? What are the
essential information for the gene information file?

When I run dChip, it gave me an error

Cannot file Affymetrix analysis call file 'E"\Graduate\STEM CELL
\Microarray folder\Heamatopoietic\GSE16589_microarray\GSM416867.txt'
No matching TXT file;calculating Presence calls...

Then an error window pop up and crashes my computer. What are the
analysis call files?

Thank you in advance for all the efforts!

Wendy