
Hi,
As you have only 3 populations (and currently gl.ibd is only running on populations) you have 3 pairwise Fsts and therefore the points are fine.
You also get the warning because the nperm setting of 1000 permutations cannot be done with 28 individuals there only the maximum number of permutations are performed in the mantel test.
If you want to do an isolation by distance on individuals you need a individual based distance e.g. 1-gl.propShared and then do your mantel test and plot “by hand”.
It is one of the things on my todo list to allow for individual and other distances.
Cheers, Bernd

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