Error when conducting gl.blast

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rebeccaloy

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Feb 10, 2025, 4:39:06 AMFeb 10
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Hi dartR team,

I am trying to conduct a blast search to align my SNPs to the Amphiprion percula genome chromosome by chromosome as follows, using the developing version of dartRverse: 

blast.gl.amphi.withperculaselectedsnps<-gl.blast(x=gl.amphi.withamphiselectedsnps, ref_genome = "Amphiprion_percula.Nemo_v1.dna.primary_assembly.1.fa")

However, I get this error:
(Repeated) Warning: Sequence contains no data
No sequences were aligned

Could I please seek your kind advice on how to correctly align my sequences? Thanks very much!

Regards,
Rebecca

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