Help on using scDaPars

[熊鸣]

I am a beginner who is interested in the phenomenon of APA. I am learning to use the package scDaPars recently. What I prepared is the 10x Genomics single-cell transcriptome sequencing data. Following steps the author suggested on the scDaPars Pipeline flow chart, a raw_PDUI matrix was calculated through the DaPars2 package. However, each column of this matrix is a sample, not a single cell. 

I check the example data, each column is the expression value of a cell. I searched for relevant information and thought that the single-cell sequencing data should be split into single-cell files based on the barcode. However, there is not much relevant information on the Internet, and I tried to use bamtools to split it and failed. Is my data analysis process wrong? Regarding the problems I am currently experiencing, can you give me some specific suggestions on the process of using scdapars? At which step should the sequencing data be split into individual cell files?

THANK YOU!

[Lometry Sun]

I had the same issue recently, I have searched online but no method works. Have you solve this problem? I will appreciate if you can share your methods!

Thanks!